Ethanol Extract Red Yeast Effect And Mechanism Of Osteoporosis In Ovariectomized Rat Model

Objective:To explore the effects of Monascus on bone mineral density,weight,OPG and ALP in ovariectomized rats.To investigate the theraputic effects and mechanicms of Monascus in postmenop- ausal osteoporosis.Methods1:According to modern pharmacological research findings,the use of ethanol solution, ultrasonic vibration,Bath nitrogen conditions to ethanol distilled to extract Monascus the active ingredientsResult1:Ethanol solution,ultrasonic vibration,Nitrogen in water bath for use rotary evaporator method can be fully dissolved Monascus powder,effective Concentration Monascus alcohol extract.Methods2:Monascus was exaracted using dissolution of alcohol and vibration of ultrasound.Thirty- two female rats were rangomly divideded into four groups,8 in each:shame group-A group,Monascus group-B group,salmon calcitionin group-c group and ovariectomy group-d group.Bone mineral densities and weights of each group were measured at 1 week,6 week and 12 week after operation.The rats were killed at 12 week.OPG and ALP were measured by enzyme-linked immunosorbent assay and ulcraviolet-visible spectrophotometry.Result2:1.BMD:①different factors of the intervention group are significantly different F=3.85 P=0.0199;Determination of the time difference was significant statistically significant F=776.79 P＜0.0001;②multiple comparisons:four groups in one weekend and six weekend overall mean no significant difference;In12 weekend there are significant differences:Group A is different from GroupB,GroupC and GroupD;Group B is different from Group A,Group C and GroupD;Group C is different from GroupA GroupB and GroupD;Group D is different from GroupA,GroupB and GroupC;2.Weight:①different factors of the intervention group are significantly different F=5.55 P=0.0041;Determination of the time difference is significant statistically significant F=254.53 P＜0.0001;②multiple comparisons:Group A is different from GroupB GroupC GroupD;Group B is different from GroupA;Group C is different from GroupA;Group D is different from GroupA;there is no different among GroupB GroupC GroupD.3.OPG:There is different between in four groups F=5.29 P=0.0051;multiple comparison:Group A is different from GroupB GroupC GroupD;Group B is different from GroupA GroupC GroupD;Group C is different from GroupA GroupB GroupD;Group D is different from GroupA GroupB GroupC;4.ALP:There is different between in four groups F=12.14 P＜0.0001;multiple comp -arison:Group A is different from GroupB and GroupD;Group B is different from GroupA Group Cand GroupD;Group C is different from GroupB and GroupD;Group D is different from GroupA,GroupB and GroupC;5.OPG and BMD of linear correlation analysis was statistically relevant difference r=0.38167 P=0.0311,there is a positive linear relationship between OPG and BMD.6.OPG and ALPof linear correlation analysis was statistically different r=-0.10034 P=0.5848,there is no linear relationship between OPG and ALP.7.BMD and ALP of linear correlation analysis was statistically relevant difference r=-0.66048 P＜0.0001,there is a negative linear relationship between BMD and ALP.8.Bone biopsy morphology:the model group femurs large disappeared marrow remnant button or rod-like trabecular bone structure,trabecular bone connecting fracture,with osteoporosis;sham group femoral trabecular bone structure showed mesh-like connecting closely with the rules,trabecular thickness consistency.Conclusion:1.Ethanol solution,ultrasonic vibration,Nitrogen in water bath for use rotary evaporator method can be fully dissolved Monascus powder,effective concentration Monascus alcohol extract.2.BMD of model was lower than that of sham-operated group,femurs large disappear,serum ALP was significantly higher than that of the sham-operated group,animal model showed reduced bone mass and bone-destroying the structure,bone metabolism high conversion rate,animal models indicate more reliable.3.The experimental results confirmed Monascus on BMD in ovariectomized rats with an increase,can effectively reduce the ovariectomized rat bone turnover rate,Slowed down due to reduced estrogen secretion caused by bone loss.Monascus can be used for postmenopausal osteoporosis prevention and treatment;s there is a positive correlation between the BMD and serum OPG level,There is a relationship between decreasement of rats bone mineral density and lowerment serum OPG levels.