Effects Of Matrine On Rheumatoid Arthritis Peripheral Blood Lymphocytes To Growth, Cell Cycle And Ifn-gamma, The Levels Of Il-4

ObjectiveTo investigate the effects of Matrine(Mat)on cell proliferation,cell cycling and cytokines of the peripheral blood lymphocyte in patients with rheumatoid arthritis.Furthermore,through contrast with methotrexate(MTX),try to find out the mechanism of treatment for rheumatoid arthritis(RA).Method1.Separate and purify lymphocyte cells.Culture lymphocyte cells outside of body.Choose Methotrexate and Matrine.2.The level of cell proliferation was measured by the MTT method.3.Cell cycle was measured by flow cytometry with PI Staining(FCM)method.4.The level of IFN-γand IL-4 was measured with ELISA.Result1.Both MTX and Mat can inhibit the proliferation of PBL in patients with RA and the effect is dose-dependent.It makes IR%increasing.(P＜0.05).IR%of 48h is more than IR%of 24h(P＜0.001).The two medicine have no obvious difference(P＞0.05).2.Both MTX and Mat can change the cell cycle and act in G₁/S point.They both make the number of cells in G₀/G₁ phase increasing and cells in S and G₂/M phase decreasing(P＜0.05). The two medicine have no obvious difference(P＞0.05).3.Level of IFN-γof Mat-treated and MTX-treated groups dropped significantly compared with control group(P＜0.001).But the level of IFN-γof Mat-treated groups is more than MTX -treated groups.All of the groups had no effect in the production of IL-4 in these doses (P＞0.05).Conclusion1.Mat inhibites the proliferation of PBL in patients with RA.The effect is correspondent to MTX.2.Mat inhibites proliferation-dependent processes of PBL in patients with RA.The effect is correspondent to MTX.3.The effect on IFN-γof Mat is weaker than MTX.4.The mechanism of Mat is similar to MTX.Mat maybe have the potential value in the treatment of RA.