Sijunzi Soup Protective Effect Of Immunological Liver Injury In Mice

Objective: To nvestigate the Modified Sijunzitang on immunologicalliver injury in mice in the treatment and to explore the mechanism of itsaction. Which observed mainly on the use of commonly used clinical liverdisease reflect gold standard: histological and reflect whether theinjury to the liver and damage to the target level of ALT and AST. Andexplore the main mechanism from the immune-pronged observation of thecompound on T-lymphocyte subsets, Natural killer cells (NK) activity,interleukin-Ⅱ(IL-Ⅱ) impact.Methods: 72 mice were randomly divided into three groups, pathology groupand the control group (-peptide), Modified Sijunzitang (large,, thelow-dose group) in all experimental mice during normal water and ordinarydiet. Exper mental start on the first day, except for the blank each tailvein inject on of saline 0.2ml, the rest of the group each tail veininjection of BCG (BCG, containing 5×107 viable) 0.2ml. Kitae day afterthe date the control group and treatment group began treatment: ModifiedSijunzitang three dose groups to the human dose of 4,2.1 times/days/only gavage, 1760mg, 880mg. 440mg/ days / only. the control group was0.2ml saline solution Kitae days / intraperitoneal injection only,control group and pathological group respectively were administrated withthe volume of saline. The experiment began 10 days. blank group to 0.2ml saline injection, other groups with lipopolysaccharide (LPS) 10ug/only the tail vein injection, injection 10 hours after treatment model:Mouse eyes were blood leaf for ALT, AST, T-cell subsets, interleukin-2testing, for the most mouse liver lobule 1cm length for biopsy, from thespleen of mice so natural killer cells (NK cells) activity assay.Comparing the biopsy group, AST, AST, T-cell subsets, interleukin-2concentration Natural killer cells (NK cells) the difference between thevitality. Result: the treatment group was superior structural pathology pathologygroup, proved Modified Sijunzitang BCG can effectively preventlipopolysaccharide in mice liver tissue structure of the immune injury.The treatment group ALT, AST than pathological indicators weresign ficantly lower Note Modified Sijunzitang effective in preventingauto mmune liver injury induced liver dysfunction. The group can increasethe CD3+, CD4+ and CD4+ and CD8+ ratio Note Modified Sijunzitang canenhance the cellular immune function, the regulation of T lymphocytesnetwork disorder, make an abnormal immune to the normal state. IncreaseNK cell activity on Modified Sijunzitang enhanced mice nonspecific immunefunction. The IL-2 treatment group was significantly higher than that ofpathological group proved Modified Sijunzitang adjust the effect ofcytokines...