Local Injection Of Vitamin C On The Effects Of Subcutaneous Adipose Tissue Morphology

Objective To observe the effect of injection with Vitamin C in inguinal fat pad of Sprague-Dawley rat,to investigate the control action of Vitamin C to adipocytes amount and volume,and to find a new method for the soft tissue of repair and reconstruction.Methods 80 Sprague-Dawley rats were randomly divided into four groups: group A(treated by 0.06%Vitamin C,24 rats),group B test solution(treated by 0.12%vitamin C,24 rats),group C test solution(treated by 0.24%vitamin C, 24 rats)and group D(blank control group,8 rats);And then each of the group A,group B and group C was randomly divided into three groups by inject time 7d,21d,35d,eight rats each group.The rats were anesthetized by intraperitoneal injection with 2%sodium pentobarbital(0.2mL/100g),and then 4ml experimental solution containing vitamin C was injected in any side inguinal fat pad of each rat while 4ml blank experimental solution was injected in the other side.Paired control was set up in the couple latuses of a rat.On the 7^thday,the 21^thday and the 35^thday specimens were taken from each rat.And then the appearance and pathology were observed;the specimen thickness,the surface density and the number density of adipocytes were measured;the amount of adipocytes and fibroblasts were count;adipocyte apoptosis was measured by TUNEL method.The numerical results were recorded as(?)±s,and relevant statistical treatment was applied.Results 1.Experimental groups of rats have no significant difference with the control group in body weight(P＞0.05).with the time continuing,the body weight is increased gradually in experimental group and the control group(P＜0.05).2.The specimen thickness(skin and subcutaneous adipose tissue)in the experimental side is less than that in the control side in group B and group C (P＜0.05).But the specimen thickness has no significant difference between the experimental side and the control side in group A(P＞0.05).The specimen thickness with the injection continuing,specimen thickness is reduced gradually in the control side in group B and group C(P＜0.05);And that of group C are thinner than that of group B(P＜0.05).3.In the experimental side of group B and group C,fibrosis is more obvious, and adipose tissue becomes thinner while adipocytes become smaller in size and decrease in amount and some cell wall is deformed and ruptured.There is no significant difference in pathology between the experimental side and the control side in group A(P＞0.05).4.On the 21^thday and the 35^thday of group C,the vascular number density is more in the experimental side than that in the control side(P＜0.05),and C₃₅＞C₂₁. There is no significant difference among the vascular number density of the other groups(P＜0.05).5.In group B and group C,the surface density and the number density of adipocytes are less in the experimental side than that in the control side(P＜0.05); There are no significant difference in the surface density and the number density of adipocytes between the experimental side and the control side in group A(P＞0.05).In group B and group C,with the injection continuing,the surface density and the number density of adipocytes is reduced gradually in the control side in group B and group C(P＜0.05);And that of group C are littlerthan that of group B(P＜0.05).6.In group B and group C,the amount of fibroblasts is more in the experimental side than that in the control side(P＜0.05),but there is no significant difference in the amount of fibroblasts between the experimental side and the control side in group A(P＞0.05).In group B and group C,with the injection continuing,the amount of fibroblasts is increased gradually in the control side in group B and group C(P＜0.05);And that of group C are more than that of group B(P＜0.05).7.In group B and group C,the amount of adipocyte apoptosis is more in the experimental side than that in the control side(P＜0.05),but there is no significant difference in the amount of adipocyte apoptosis between the experimental side and the control side in group A(P＞0.05).In group B and group C,with the injection continuing,the amount of adipocyte apoptosis is increased gradually in the control side in group B and group C(P＜0.05);And that of group C are more than that of group B(P＜0.05).ConclusionsLocal injection with experimental solution containing 0.12%and 0.24%vitamin C in adipose tissue can promote adipocyte lipolysis and adipocyte apoptosis,and decrease in the amount of adipocyte.Meanwhile it can promote fibroblasts increase and the effect is obvious correlative with concentration and time.But injection with the experimental solution containing vitamin C0.06%do no significant impact on adipose tissue morphology.