| Chinese medicine regulation mechanism of the human body has its own understandingof the theoretical system and effective means of regulation, such as "the unityof opposites of yin and yang theory, the theory of five elements theory, theancients used these macro,movement changes thinking to understand and treatdiseases. As soon as nerve - endocrine - immune regulation theory beendiscovered,pepoles realized that they have many similaties, particularly in themedical understanding of the five internal organs function and West respiratorymedicine, sports, genitourinary, digesting, and the circulatory system .so itprovide us a good link and bridges to research."Lung corroesponds to the Autumn "as the theory of Chinese medicine is animportant component of the theory of Chinese medicine in the Habitat importantposition. "Lung corroesponds to the Autumn "adaptive control theory refers tothe fall of the lungs should be able to adjust their own environmental changeand the relationship with other viscera, in order to maintain the environmentfor the steady-state, its regulation and control methods and neural - endocrine- closely related to immunization. Therefore, this issue from the body's immunedefense and lung function as the breakthrough point, from the pineal gland - therelationship between melatonin and lung research in the lung and the same shouldbe the fall-immune material basis and from the perspective of macro-andmicro-immune Functional changes in the law.1.Objective"Pulmonary corroesponds to the Autumn" adaptive control mechanism for theresearch object, meaning its theoretical analysis and experimental results ofthe summation of Chinese Zang-Xiang of the nature of research like thinking andresearch methods. By comparing the four seasons and differences in the levelsof melatonin secretion and immune function changes in a coherent exposition"pulmonary corroesponds to the Autumn" nature of the regulation.2. Methods2.1 Theoretical methodsThe literature and experimental research methods, the Chinese " Pulmonarycorroesponds to the Autumn " control mechanisms of TCM theoretical connotationand modern nerve - endocrine - Immune Network's overall regulation theory, theessence of Chinese Zang-Xiang as empirical studies suggest the feasibility ofideas and channels, and Experimental study on the topic provide some new modeland reference. 2.2 Experimental methods(1) Experimental ideas: The experimental study " Pulmonary corroesponds to the" related nerve - endocrine - immune axis as a starting point, select the pinealgland removed animal model as study targets, a pulmonary and humoral immunityas a related molecule detection object,the rats were simultaneously observed inthe physical condition after removal of the pineal gland and the immune parameterschange, and observe the pineal gland in the course of high regulation.(2) Animal: Male Wistar rats, 180-200g. Physiological were randomly divided intogroups, pseudo-operation group, operation group. Natural light, free intake diet,keep at room temperature. Rats from Beijing Lihua-dimensional experimentalanimals Limited company, 40 days before the Solar Terms, a week later, keepingthe pineal gland removal surgery (surgery), pseudo-operation group only vectorvein ligation pineal but not removed gland, rats were bred after the operationand were decollated at night on the Spring equinox,Summer Solstice,Autum equinoxand Winter Solstice.(3) Detection Methods:①lavage fluid in sIgA immunoglobulin, interferon-γ(INF-γ): ELISA method;②serum melatonin and lung tissue of melatonin: Theenzyme-linked immunosorbent assay.③serum IL-3:enzyme-linked immunosorbentassay; IgG Determination: immune Turbidimetry.(4) Analysis: All data are used SPSS11.5 statistical software for analysis.Multi-group comparison with analysis of variance,linear correlation betweenvariables.3.Results3.1 Resultus of theoretical study(1) Ancient physicians on human life through the activities of the long-termobservation, in the ancient philosophy of Yin and Yang and the five elementstheory "and the world of the Senate," inspired by the view, the inner lives ofpeople recognize activities and the natural environment, social environmentexists between interrelated, mutual checks complex relationship. Five Viscerasystem of the human body through the activities of the self-regulation function,which adapt to the changing external environment.(2) "Pulmonary corroesponds to the Autumn" comes from "HuangDi NeiJing" in the"organ law" theory, has a profound historical background and culturalconnotations. It holds that, in the nature of human existence, its internal organsof the physiological function inevitably be outside the natural rise and fallof the yin and yang from the impact of the corresponding change. Based on this,"the lung corroesponds to the Autumn" refers to the physiological function ofthe lung with the fall of climate change and show a corresponding variation of the main pulmonary gas, the Secretary breathing, clear water diversion Road, themain features such as fur show in the autumn climate adapt to changes in theadaptability of regulation, in order to preserve the environment for thesteady-state, to balance Yin and Yang.(3)Large number of studies confirmed that in the corresponding dirty with thedirty on the season and the adaptability of the mechanism of control, with theseasons change pineal gland secretion of melatonin and is the medical dirty withthe corresponding principal regulator. On the one hand, its natural signal factor- a light sensitive reaction in the light conditions, the pineal gland secretionof melatonin decreased in the dark conditions, increased secretion. At the sametime it is important neuroendocrine transducer, as a high neuroendocrineregulation organs, the immune system has an important role.3.2 Results of experiments3.21 Seasonal variation of serum and lung tissue of melatonin.①spring, physical group and pseudo-operation group more significantdifference,P<0.05(P=0.04).Summer pseudo-surgery group and physiological groupsand the group has significant difference, P<0.05.Serum melatonin, whetherphysical group, pseudo-operation group, surgery group that spring equinox>WinterSolstice> Autumnal Equinox>summer solstice changes in the trend. And the springequinox> Autumnal Equinox, Winter Solstice> summer solstice. And the pineal glandremoved after each season melatonin content decreased.②melatonin lung tissue, whether physical group, pseudo-surgery group thatspring equinox> Winter Solstice> Autumnal Equinox> summer solstice changes inthe trend. And the spring equinox> Autumnal Equinox, Winter Solstice> summersolstice. And the pineal gland removed in addition to the summer after the otherthree quarters of melatonin content decreased.3.22 Seasonal changes effect the Systemic Immune and pulmonary to systemicimmune.①IFN-γlevels in each group there was no significant difference, but we can seea trend, that is, in addition to surgery group than in spring, summer solsticelow, and the other group with its seasonal variations in the content of alveolarshowing a downward trend, Spring > summer> autumn> winter.②Spring equinox in rat alveolar lavage fluid sIgA pseudo-surgery group andphysiological groups and the group there were significant differences (P <0.05),Autumnal Equinox surgery group and the group and pseudo-physiological differencebetween the surgery group and significantly (P <0.01). Throughout the spring andsummer showing a higher level contrast to a lower level of autumn and wintertrends. ③index seasons rat thymus, spleen showed the index for the spring and summerand autumn and winter high-low, the two changes is the same.④This case found that the rats physiological levels of IL-3 in the winter showedthe upward trend,the difference is significant(P<0.01), indicating that in thewinter the body of the hematopoietic function is enhanced, with Chinese medicine"kidney to Winter "consistent with the theory. Pineal gland removed, IL-3 wassignificantly decreased, the melatonin for the proliferation of IL-3 has animportant influence.⑤Rat IgG changes in the content of the performance of rough-spring and autumn,summer and winter increased lower pineal gland resection, lower IgG response wasthat the secretion of melatonin secretion reduce IgG had an impact.3.23 Pineal gland in rats of various immune indicatorsAfter resection of the pineal gland,lung tissue and serum melatonin secretiondecreased significantly and the rhythm of the disappeared. Immune function inaddition to showing a lower in autumn and winter than in spring and summer, alsofound that black and push-secretion coupling relationship exists. That the thymusindex,spleen index,SigA,serum IgG and so IFN-γsecretion of melatoninperformance consistent with the trend of changes. Pineal gland resection, suchtrends do not change significantly, there was no significant difference betweenthe two groups. This can to some extent that the body of the pineal gland as ahigh regulate organ, through its secretion of rhythm changes the immune statusof impact.4. Conclusions4.1 Chinese " Pulmonary corroesponds to the Autumn " has the basis ofimmunological substances.The topics start from the point of view of immunology, through animalexperiments, we found that the immune function of the body and lung there is atrend of seasonal changes, most of immune performance indicators increased inspring and summer, decline in autumn and winter. Lung actively adapt to theenvironment and climate change to a corresponding internal mechanism to vent thepulmonary function decline in the autumn enhanced role Su drop as a result ofreduced immune function of the main factors. It is fact that the changes in immunefunction has a close relation to the "lung corroesponds to the Autumn".4.2 "Pulmonary corroesponds to the Autumn" has closely relation to thehigh-conditioning of Pineal gland.This study shows that normal rat serum and lung tissue Melatonin in thesunshine with the change of time and showing a corresponding change in the law,with obvious light-dependent characteristics. After resection of the pineal gland, lung tissue and serum melatonin secretion decreased significantly and therhythm of the disappeared. Immune function in addition to showing a low in autumnand winter but high in spring and summer, we also found that black andpush-secretion coupling relationship exists. That the thymus index, spleen index,SigA, serum IgG and so IFN-γsecretion of melatonin performance consistent withthe trend of changes. Pineal gland resection, such trends do not changesignificantly, there was no significant difference between the two groups. Thiscan to some extent that the body of the pineal gland as a high regulate organ,through its secretion of rhythm changes and the immune status of impact.4.3 "Pulmonary corroesponds to the Autumn" adaptive control mechanism.Through this study, and preliminary experimental results, we believe thatthe initial "pulmonary corroesponds to the Autumn" in the immune regulationmechanism is as follows: seasonal light changes will be a continuing stimulussignals through the optic nerve transfer to the retina of the hypothalamussuprachiasmatic nucleus, Then sympathetic postganglionic fiber will be conveyingthis message to the pineal gland, thereby enabling a pineal gland secretion MTrhythm changes. MT is released into the blood through the organ. Throughactivation of the immune system in two ways; (1) the role of endogenous opioidpeptides in the system and, indirectly, activates the immune system, leading toimmune enhancement. (2) a direct role in the immune organs (such as the thymus,spleen) and immune cells (such as monocyte IL-2 and IL-6 expression) play itsrole in immune regulation. In the autumn, because of the shortened cycle of light,resulting in the pineal gland secretion MT decline, and that the rhythm of changesto the body's immune cells to produce sustained impact, so that the immunefunction in the autumn performance of "convergence 'phenomenon. |
