Traf In-6 Expression In Lps-mediated Human Periodontal Ligament Fibroblasts Inflammatory Injury

[Objective]: To investigate the gene and the protein expression of Tumor Necrosis Factor Receptor-associated factor-6 (TRAF-6) in human periodontal ligament fibroblasts (HPLFs) after the intervention with different concentrations of Lipopolysaccharide. To provide experimental basis for periodontal disease prevention, diagnosis and treatment.[Method]: Normal human periodontal ligament cells were isolated and identified in vitro. In order to establish the inflammatory damage cell model, four different concentrations (0.1ug/ml,1ug/ml,10ug/ml,100ug/ml) of LPS were used to intervene normal periodontal ligament fibroblasts. By Reverse Transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry methods in the combination with the computer image analysis method, we observed the expression and localization features of TRAF-6 in different concentrations of LPS intervention. Hereafter, we explored the relationship between TRAF-6 and the injury of LPS-mediated inflammatory periodontal ligament fibroblasts, and further investigated the role of TRAF-6 in the process of periodontitis mechanism.[Result]: (1) RT-PCR results showed that non TRAF-6 is expressed in normal human periodontal ligament fibroblast. With the increasing concentration of LPS from 0.1 ug/ml to 100ug/ml, TRAF-6 expression increased first and then gradually decreased. (2) Immunohistochemical results were consistent with RT-PCR results .[Conclusion]: This study has indicated that on the molecular level, TRAF-6 is an important signal transducer which is involved in HPLFs damage process. It suggests that TRAF-6 may have very important function during the LPS-mediated signal -regulated revolution and helps to improve the research about LPS-mediated signal-regulated network. This study also provides a theoretical basis for the treatment of LPS caused the acute inflammatory response and adds new information for the periodontal ligament fibroblasts cytokines controlled network. Furthermore, it provides experimental basis for further in-depth study about TRAF-6 on the periodontal ligament fibroblasts factor controlled network. It may help provide new ideas for the diagnosis and treatment of periodontal disease.