Curcumin Extraction And Purification, Preparation Of Liposomes And Tissue Distribution Studies

Curcumin has indicated that curcuminoids,the main activeingredient in curcuma,has significantly inhibitory effect on varioustypes of tumors.It can influence each pHase of the tumorgrowth(initiation promotion and progression)and has inhibitory effecton infiltration and metastasis by means of inhibition the tumorangiogenesis.However, curcumin can not be dissolved in water and canalso be oxidized easily in vitro,there has been no drμg preparationwith good stability.Besides,In vivo studies have revealed that smallamount of curcumin can be detected in plasma after oral administration.These bring about difficμlties in the clinical application of curcumin.Recent studies have shown that liposome encapsμlation technique can beemployed to solve the problem that fat-soluble drμgs Ean not be easilydissolved in water.Liposome is also an ideal carrier of anti-tumordrμgs.It can decrease the toxicity of the encapsμlated drμgs andincrease the amount of drμgs absorbed by highly proliferative tumorcells.Therefore,We study The curcumin 's extraction and purification,separation,also the curcumin liposome in the preparation and distribution of animal behavior.The purpose is to exploring the possibility of new preparation ofcurcumin suitable for venous administration using liposome as a carrierand further understanding the effects of curcumin on the biological properties and to provide proof for the further studies.Methods and resμlts1 Using HPLC(HPLC)Determinate the Content of curcumin study the establishment of standards,and methodology inspection.2 On the stability of curcumin research,research shows that curcumin in PH＜5.5,the temperature at 30℃,curcumin has not changed the basic content,when the temperature is above 40℃,curcumin in decline.3 Leakage from the curcumin,the research determine the best extraction process for: PH=5.5,20 times the volume,75 percent of ethanol,soaked for 10 hours after the leakage flow rate was adjusted 1 mL/min for leakage.4 In this paper,DA201,DS401,D101,D101A,DM301 five macroporous resin filter.The resμlts showed that,DM301 resin for the absorption of curcumin reconciliation better absorption rate to determine DM301 for the purification of curcumin resin.DM301 macroporous resin purification conditions for the conduct of studies to determine the best conditions for purification.5 Column by separation and purification of curcumin,the monomer be successfμl.6 Select films,reverse evaporation,ethanol injection method,ether and melt into law prepared by curcumin lipid Body to encapsμlation efficiency as the main indicators, combined with microscope to determine the optimal method for thin-film method.7 Single factor to study the best optimization of technology;determine the best technology for:lecithin and cholesterol ratio of 3:1,pHospHate buffer salt solution to the pH 5.5,the ion concentration of 0.01 moL·L^-1,Amount of 30 mL.Curcumin amount of 1 mg.8 With a homogeneous Machine AH110D the liposomes curcumin,found that curcumin liposome size smaller.9 Curcumin liposomes to the initial pHysical and chemical parameters of the study, using transmission electron microscopy techniques observe the appearance of a complete round or oval-shaped spHerical particles,mainly distributed in diameter around 33.3-88.7 nm,measured using electropHoresis Surface charge for the negative charge,pH 4.8 LDC 0.2,the rate was 73.49 percent encapsμlation,the best storage conditions are 3-5℃Cold Storage,leakage rate of 0.27%10 In this paper,the curcumin tail vein injection of liposome delivery in mice tissue distribution,the resμlts showed that curcumin liposomes in the liver,lungs and other organs and the distribution of moreConclusion1,Using HPLC Determination of the content of curcumin,the method is accurate and reliable,convenient and feasible.2,Leakage from curcumin,the method is simple and convenient,its industrial production provides a basis.3,DM301 resin purification curcumin simple and feasible,but have yet to be further transfer rate of curcumin. 4,The film prepared by curcumin liposome method is simple,encapsμlation efficiency high.5,The choice of curcumin as a liposome drμg carriers is feasible,curcumin liposome encapsμlation high rate of drμg solubility increased significantly,enhancing stability.6,Liposomes curcumin in mice distribution experiments show that curcumin liposome injection than the free distribution of Red injection quickly,lasted for a long time,strong pHarmacological effect,eliminate slow.Curcumin liposomes in the liver and lung,and other organizations in the distribution increased significantly and is conducive to the targeting of curcumin.