Chronic Compound Stress On The Fertility Of Male Mice And Chinese Medicine Intervention Role

ObjectiveThe investigation on the reproductive capacity of male mice in chronic compound stress ofthe different time and the influence of different herbs.Methods1. 100male Kunming male mice were randomly divided into six groups: three-week normal group; three-week stressed group; five-week normal group; five-week stressed group; seven-week normal group; seven-week stressed group. five stress methods : psychological stress, restraint, tail suspension, water platform environment and clamping tail battle. control different stress time to the make chronic compound stress model. get the serum and test the contents of the FSH,LH and T,remove and weigh the testics and the epididymis,carry out the sperm count, image(quality analysis of the sperm)and measurement of intracellular PH in spermatozoa. Testicular slice and analyze their pathological change.2. 60 male Kunming mice were randomly divided into four groups. Random arrangement this five stress methods of psychological stress, restraint, tail suspension, water platform environment and clamping tail battle into the different stress time to make chronic compound stress model and feed the mice with herbs during the whole stress period. Test the features of the mice reproductive capacity.ResultsThe way of the chronic compound stress can degrade the grouth rate, the sperm count andthe motility rate of the mice significantly. intracellular PH in spermatozoa and the content ofthe FSH and T step down differently. Different herbs have different influences in the recoverof the mice reproductive capacity.ConclusionsThe way of the chronic compound stress which is made of five stress methods can changethe physiological nature of the physiological nature significantly,reduce the fertility. With theprolongation of the stress time, there were eversible changes in part of the features. With theinfluence of the herbs, the features of the mice reproductive capacity recovered differently.