| Objective To explore the cardio-protective effects of the adenosine A1/A2 receptor agonist 5'-N-ethylcarbo-xamidoadenosine (NECA) on acute ischemic/reperfusion in rabbits, and it's possible mechanism.Methods 64 New Zealand white male rabbits were randomized into four groups: (1) control group(n=16),without any intervention; (2) postconditioning group (Post-con group, n=16), At the first three minutes of reperfusion, three cycles of 30 s reperfusion/30 s ischemia preceded 3 h reperfusion; (3) Adenosine 5'-triphosphate postconditioning group (ATP-post group, n=16), ATP (300 ug·kg-1·min-1) was used at 5 min before reperfusion and lasted 1 h; (4) NECA postconditioning group (NECA-post group, n=16), NECA(0.2 ug·kg-1·min-1) was used at 5 min before reperfusion and lasted 1 h; Each group was divided into two subgroups: Group A (n=8) and Group B(n=8). Acute ischemic reperfusion model was created, group A: 40 min coronary occlusion was followed by 1 h full reperfusion; group B: 40 min coronary occlusion was followed by 3 h full reperfusion, von Willebrand factor(vWF) in blood sample and the gene expression of vWF in myocardium was measured in Group A. Infarct size and the plasma CK-MB was measured in Group B.Results (1)In NECA group, myocardial infarct size[(13.94±1.53)%vs(18.75±1.19)%, p<0.05] was limited and the level of CK-MB[(241.43±7.82)u/l vs (349.21±8.73)u/l, p<0.05] was lower than those of control group, and it was consistent with the ATP-post group and POST-con group; (2) Compared with control group, vWF in blood sample at reperfusion after 1h and the gene expression of vWF in myocardium were significantly lower in NECA group [(0.74±0.20)%vs(0.87±0.24)%; ( 133.58±1.98)%vs(143.21±2.81)%; P<0.05], and it had no difference with ATP-post group and POST-con group.Conclusions NECA can reduce infarct size and protect rabbit heart from reperfusion injury, it's mechanism may be associated with alleviating endothelium injury by activation of adenosine receptor. |
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