Hopane Bile Pill And Its Raw Materials Of Patchouli Leaf Pharmacological Research

HuoDan pills are traditional Chinese medicine composed of GuangHuoXiang leaf and ZhuDan powder.It's use to cure chronic rhinitis and chronic paranasal sinusitis.It has significant and clear effect.But it's the blank concerning the pharmacological research of HuoDan pills. It's no relevant reports concerning the pharmacological research of the different chemical location home and abroad,especially,the pharmacological mechanism of action of HuoDan pills. To interpret the relative pharmacologic action of HuoDan pills, analyze the effective chemical location of HuoDan pills, identify the resource of crude drug huoxiang leaf.1 The pharmacologic action research of HuoDan pills1.1 The anti-inflammatory effect of HuoDan pillsTo study the anti-inflammatory effect by ear swelling of mice caused by topical smearing of xylene﹑forced foot of rat by CAR and agar granulation tumor model of rat;HuoDan Pills could markedly inhibit dimethylbenzene-induced ear swelling in mice; significantly inhibit the swelling of foot paw induced by Carragenin and the granuloma induced by Agar in rats,so HuoDan pills have anti-inflammatory effects.1.2 The analgesic effect of HuoDan pillsTo study the analgesic effect by writhing reflex induced by intraperitoneal injection of acetic acid in mice served as models of inflammation and sensation of pain in the mice.HuoDan Pills could relieve pain of mice;so HuoDan pills have the analgesic effect.1.3 The immunifaction of HuoDan pillsTo study the immunifaction by the producing of the amount of haemolysis plain antibody in the mice body and carbon particle clearance test.HuoDan pills can increase the content of mice serum hemolytic and enhance immunocompromised mice monocyte-macrophage phagocytosis.So HuoDan pills can enhance the immunifaction.1.4 The bacteriostatic action of HuoDan pillsTo investigate the bacteriostatic effect of staphylococcus aureus﹑ hemophilus influenzae﹑ streptococcus pneumoniae and catarrhal coccus in vitro and staphylococcus aureus in vivo. HuoDan Pills could inhibit bacteria in vitro.1.5 The anti-anaphylaxis effect of HuoDan pillsTo study the anti-anaphylaxis effect by the increase of abdominal cavity capillary permeability and dermato-allergic reaction of mice due to antihistamine.HuoDan Pills could elevate the sensitization threshold induced histamine and inhibited histamine-induced skin capillary permeability-increasing.So HuoDan pills could inhibite the hypersensitiveness.1.6 The anti-inflammatory mechanism of action of HuoDan pillsTo detect the prostaglandin and histamine and malonaldehyde in the mice's inflammatory tissue and approach anti-inflammatory mechanism.HuoDan Pills could reduce the content of prostaglandin and histamine and malonaldehyde;the anti-inflammatory mechanism of action relate to reduce the content of prostaglandin and histamine and malonaldehyde.2 The pharmacologic action investigate of different chemical location of HuoDan pillsTo study the pharmacologic action investigate of different chemical location of HuoDan pills by ear swelling of mice caused by topical smearing of xylene and writhing reflex induced by intraperitoneal injection of acetic acid in mice served as models of inflammation.It adopt orthogonal design to decide the groups and the dose.The inter-compatibility of the aetherolea of huoxiang leaf,the aqueous extract of huoxiang leaf and the alcohol extract of ZhuDan powder could lower swelling of the mice in auricular swelling test,decrease the stretching frequency,besides simple use the aqueous extract of huoxiang leaf and the alcohol extract of ZhuDan powder.But the anti-inflammatory interaction of the aetherolea of huoxiang leaf and the alcohol extract of ZhuDan powder is significant.Perhaps the aetherolea of huoxiang leaf is the effective chemical location.3 Compare the chief pharmacodynamics of the different area Huoxiang leafTo study the effect on suppressing inflammation and alleviating pains of the different area Huoxiang leaf by ear swelling in mice caused by topical smearing of xylene and writhing reflex induced by intraperitoneal injection of acetic acid in mice served as models of inflammation and sensation of pain in the murines.To study the effect on anti-hypersensitivity by the inhibitory actions of it on the increase of abdominal cavity capillary pemeability in mice due to histamine.To investigate the bacteriostatic effect of the different area Huoxiang leaf on the antibacterial activity of staphylococcus aureus﹑ hemophilus influenzae﹑ streptococcus pneumoniae and catarrhal coccus in vitro and staphylococcus aureus in vivo.PaiXiang and NanXiang could inhibit dimethylbenzene-induced ear swelling in mice,prolong the initiatre of pain caused by heat irritation,decreasethe rolling reaction times of mice,lower abdominal cavity capillary pemeability in mice due to histamine. It was shown that the different area Huoxiang leaf,to some extent,exerted bacteriostatic action on staphylococcus aureus﹑ hemophilus influenzae﹑ streptococcus pneumoniae and catarrhal coccus.PaiXiang of Staphylococcus aureus in vivo inhibition in mice showed increased survival rate trend.The different area HuoXiang leaf has the significant effect on anti-inflamatory﹑alleviating pains﹑anti-hypersensitivity and bacteriostatic action.