Study On Chemical Composition Separation And Pharmacological Action Of Phyallanthus Urinaria L.

The purpose of this paper was to discsuss that analysis and identificate separation by using HSCCC and HPLC, in order to establish a less pollution, high recovery and high-efficiency technology processe. It also researched pharmacological action of Phyllanthus urinariu L. with a view to provide theory for further development and application. It may be summarized as follows:1. Separation and purification of compounds from Phyallanthus urinaria L. by HSCCCObjective: Separation and purification of compounds from Phyallanthus urinaria L. by HSCCC. Methods:The TLC technique was firstly used to examine the partition of target compounds between the two solvent phases , primary choose the solvent systems of HSCCC . Sencondly by analytical HSCCC to further choose the solvent systems : chloroform-methanol-water, and the preparative separation was then performed by adjusting the flow rate. Results: By analytical HSCCC, the extract of ethyl acetate, 150 mg, was separated with a two-phase solvent system composed of chloroform-methanol-water(4:3:2), produced 2 mg of target compound 1 with putity of 80.7%. The extract of water, 150 mg, was separated with a two- phase solvent system composed of chloroform- methanol-water(4:3.5:2), produced 1.8 mg of target compound 2 with putity of 90.2%. Target compounds could be separated by HSCCC. Conclusion: It had completely separated target compounds form the ethyl acetate and water parts by this solvent system.2. Antibacterial activities in vitroObjective: Research its antibacterial activities in vitro. Methods: The antibacterial activities of the extracts of the Phyallanthus urinaria L. were tested against S.aureas,E.coli. The herbal was extracted by water,75% ethanol,95% ethanol. The extract of the 95% ethanol was again extracted by petroleum ether,chloroform,ethyl acetate. The extracts were tested for the antibacterial activities by the digging holes method. MIC also was tested by test tube method. Studied on antimicrobial mechanism by using scanning electron microscope. Results:It showed that the herbal had antibacterial effect to S.aureas,E.coli. The MIC of S.aureas was 31.25 mg/mL. The MIC of E.coli was 62.5 mg/mL. In the paper, the antibacterial mechanism of the herbal was studied. The extract of water could inhibit cell division at logarithmic phase. The result of scanning election microscope revealed that the E.coli. had been constricted and distorted. Conclusion: The herbal had obviously antibacterial activities.3. Antioxidant activity in vitroObjective: Research its antioxidant activities in vitro. Methods: In this research, The herbal was extracted by water, 75% ethanol, 95% ethanol. The antioxidant properties were evaluated by determining the ability of the extracts solution to scavenge hydroxyl, superoxide radicals and inhibit the level of hemolyse of mouse induced by hydrogen peroxide. Results: When the concentration was1mg/mL, the ability of the extracts by water was greatest which was 90.9%,62.5%,20.5%, and the extracts by 75% ethanol was 35.6%,40.2%,16.7%, the extracts by 95% ethanol was 48.4%,21.3%,17.8%. Conclusion: The herbal had obviously antioxidant activity obviously.4. Liver-protect activity in vitroObjective: Research its liver-protect activity in vitro. Methods: The liver cell from Oreochromis niloriczcs was cultivated in vitro, to establish in vitro CCl₄ experiment of liver cell culture. The herbal was extracted by water, 75% ethanol, 95% ethanol, and the paper researches these extracts' affections on ALT and AST. Results: The content of ALT and AST was in the drug groups were lower than the damaged group. Conclusion: In this test the extracts had obviously protective activity.