Model Of Irritable Bowel Syndrome And Tong Xie Yao Fang Blood And Colon Tissue Of Vip, Ss Affect Experimental Research

Objective:Sets up a irritable bowel syndrome model, and Tongxiey- aofang is used to research effect of vasoactive intestinal peptide and Somatostatin in the artery and Colon of irri- table bowel syndrome model.Methods:Totally 42 neonatal SD rats is selected. With help of adopting by chronic stimulation rats in colon and on tail to sets up the model of intestinal hypersensitivity and to evaluate model .After verification establishing model successful, totally 120 neonatal SD rats is anew selected and that were randomly divided into six groups: the addition of the normal control group (Group A),the model group(Group B),the low dosage of Tongxieyaofang group (Group C),the middle dosage of Tongxieyaofang group (Group D),the high dosage of Tongxieyaofang group (Group E), with the Spasmomen control group(Group F).Treatment of IBS model rats by tongxieyaofang,and compare with Spasmomen,to observe effect that is vasoactive intestinal peptide and Somatostatin in the artery and Colon of model rats.Results:(1) In contrast to neonatal rats subjected to saline intrarectally (A group) and neonatal rats subjected to acetic acid intrarectally (B group), neonatal rats subjected to acetic acid intrarectally and stimulated with forceps clip on tails (C group) showed a significant decrease in the volume threshold of abdomen lifting and body arching during rectal distension(P<0.01). (2)There was a significant increase of abdominal electrical activity responding to 0.3, 0.6, and 0.9mL distention volume. (Compared with B group P<0.05, P<0.05 and P<0.05, respectively. Compared with A group P<0.01, P<0.05 and P<0.05, respectively). (3) model of plasma in the colon and VIP and SS were significantly increased, high-dose pain, diarrhoea and western medicine to Siba Min can significantly reduce plasma and colon SS concentration (P <0.05), pain, diarrhoea to low-dose Group, the dose effect not significant.Conclusions:(l) by IBS rat model behavior, physiology, pathology section of colon and indicators of the observation that: chronic colon and stimulate the end folder to stimulate the establishment of the method of combining the high sensitivity of intestinal IBS model can better simulate the etiology and pathogenesis. (2) High-dose pain to diarrhea side IBS model of plasma and colon in VIP, SS was reduced, it tends to be normal, the treatment of irritable bowel syndrome in one of the mechanisms.