The Chemical Composition Of The Mileensis And The Revised Standard 2010 Version Of "chinese Pharmacopoeia" Mileensis

Mile Swertia Herb, a traditional Chinese drug "Qingyedan", is the dried herb of Swertia mileensis T. N. Hoet W. L. Shih, which belongs to the Fam. Gentianaceae. Qingyedan is bitter in taste and slight cold in nature. The drug mainly distributes in the Yunnan Mile and Kaiyuan County, et al.. It can increase the flow of bile, and remove heatdampness. Collected in Chinese pharmacopeia, the drug is used for the treatment of tanudice with dark urine, acute urinary infetion with difficult painful urination.Flavonoids, iridoids, xanthones and triterpenoids are considered as the predominant ingredients in Mile Swertia Herb. In many related reports, the effective components have considerable activities such as liver protection, spasmolysis, analgesia, anti-inflammatory, anti-virus, etc.. In this research, Mile Swertia Herb was collected, cut into pieces and dried. Then the drug was immersed with 95% ethanol, after removing the solvent, the ethanol extracts were dispersed into water, extracted with dichlormethane and n-BuOH saturated with water in sequence. The n-BuOH extracts were subjected to silica gel column chromatograph and sephadex LH-20 column chromatograph to obtain four compounds. Through measuring their physical and chemical constants and spectroscopic analysis (UV,IR,¹³CNMR,¹HNMR) , four of the structures were elucidated as swertiamarin , gentiopieroside , oleanolic acid and isovitexin. The isolation and identification researches into chemical constituents of Mile Swertia Herb have provided bases on chemosystematics of species swertia herb, the relevant study of genetic relationship and utilization of plant resources.On the basis of obtaining the components mentioned obove, the author developed a quick and accurate capillary electrophoresis method for the quantitative analysis of the compuonds by studying the migration behavior of swertiamarin and isovitexin on high performance capillary electrophoresis (HPCE). According optimising all the factors involved, we found that the power of hydrogen, concentration of buffer solution and the separation voltage have a significant impact on separate effect. The method is highly sensitive and rapid with good stability. Compared to HPLC, HPCE has the quality of satisfactory separation speed, less sample consumption, environment friendly and low cost. It can sever as a rapid and effective analytical method for the quality control of Mile Swertia Herb. Meanwhile, we assumed one standard research project of Pharmacopoeia of People's Republic of China Edition 2010: Quality specification revision of Mile Swertia Herb. In this part, HPLC method was adopted to determine the contents of swertiamarin and gentiopieroside in medical material and decoction pieces of Mile Swertia Herb of different habits. Simultaneously, the content limitation was established. In addition, the description, microstructure, TLC, water content, ash content and acid-insoluble ash content of Mile Swertia Herb were researched. All these studies have shown significant importance to evaluate the quality and sufficiently utilize the resource of Mile Swertia Herb. The check of the experimental results now is being carried out by Yunnan Institute For Food and Drug Control.