Laurencia Terpenoid Alcohol Exposure To Serum Lipid And Antioxidant Levels

Objective: To study of laurencia extrac (LET) on lipid lever and antioxidant system of rats after alcohol exposure.Methods: 1.Sixty male Wistar rats were randomly assigned to six groups: Group A (blank group) was orally given with distilled water; Group B was orally given with 50% alcohol of 4.8 g/kg·d; Group C, D and E was orally supplemented with LET of 25, 50 and 100 mg/kg·d respectively; Group F (positive control) was fed with diammonium glycyrrhizinate of 200 mg/kg·d. All rats were treated for 6 weeks. Six weeks later, the rat blood and hepatic tissue were collected.2.The hepatic ultra-structures were studied.3.The lever of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyeride (TG), cholesterin (CHO) in plasma and the activities of alcohol dehydrogenase (ADH) in hepatic tissue were measured by biochemical methods with kits.4.The activities of superoxide dismutase (SOD), glutathione-peroxidase(GSH-Px), the content of malanydiadehyde (MDA) in plasma were measured by biochemical methods with kits. The expression of heme oxygenase-1 (HO-1) in hepatic tissue was measured by immunohistochemistry assay.Results: 1.The hepatic ultra-structures result showed that alcohol exposure led to hepatocytes steatosis, slight lymphocytes infiltration, mitochondria swollen deformative and rough endoplasmic reticulum degenerative, collagen fibers accrementition around blood sinus. Groups supplemented with LET or diammonium glycyrrhizinate showed slight destroy degree.2.Compared with blank group, the contents of ALT and AST in plasma of B group increased (P<0.05). Compared with model group, the contents of ALT in plasma of E, F groups decreased (P<0.05); the contents of AST in plasma of C, D, E ,F group decreased (P<0.05). The activity of ADH in hepatic tissue of A group was higher than other groups. Compared with blank group, the activity of ADH in B group decreased (P<0.05).Compared with blank group, the lever of TG in plasma of B group increased (P<0.05); compared with model group, the lever of TG in plasma of C, D, E, F groups decreased (P<0.05). Compared with blank group, the lever of CHO in plasma of B group decreased (P<0.05).3.Compared with blank group, the activity of SOD in plasma and the GSH-Px in hepatic tissue of B group decreased (P<0.05); the contents of MDA in plasma and hepatic tissue of B group increased (P<0.05). Compared with model group, the activity of GSH-Px in plasma of D group increased (P<0.05); the contents of MDA in plasma of C, D, E groups and in hepatic tissue of E, F groups decreased (P<0.05). HO-1 was expressed in liver cells surrounding central veins of hepatic lobules. Compared with blank group, the expression of HO-1 in B group was decreased (P <0.05). Compared with model group, the expression of HO-1 in E, F groups were increased (P <0.05).Conclusion:1.LET at certain dosage could promote mitochondria hyperplasy, to accelerate accretion rate, to improve hepatic ultra-structural pathological change in rats after alcohol exposure.2.Intervention of LET could reduce the degree of injury of hepatic cell envelope, depress the lever of transaminase in serum and regulate lipid lever to relief lipid metabolic disturbance evidently.3.LET can protect against oxidant injury after alcohol exposure, which may be associated with raise activity of antioxidant enzyme, reduce lipid peroxidation, and induce expression of HO-1 enhancement in liver cells.