The Inhibitory Research About Three Components Of Astragalus On Endometrial Cancer Cell Line KLE

Objective:To study Total Astragalosides (TA),Astragalus Polysaccharides(APS), and Total Flavonoids of Astragalus (TFA), alone or in combination with cisplatin on the growth of endometrial cancer cell line KLE.Looking for which kind of composition play the most important role about Kill the cancer cells.Methods:KLE cells were treated by TA, TFA or APS at different concentrations. Cells growth and proliferation were observed by Neutral Red assay. Morphological changes of cells were observed. Apoptosis and cell-DNA distribution was analysized by flow cytometry. The changes of programmed cell death5(PDCD5) expression in TA group KLE cells were detected by immunofluorescent. Finally, we use a factorial design of F test method of experimental data for statistical analysis.Results:1.TA could inhibit KLE cell’s proliferation. KLE cells viability in presence of TA decreased markedly in a dose-and time-dependent manner, and the joint effect of the two drugs had asignificantly higher inhibition rate, showing their synergistic effects. Flow cytometry revealed that TA could cause G1phase arrest and induce apoptosis at the same time. This effect may be realized by raising the expression of PDCD5in cells.2. APS could inhibit KLE cell’s proliferation. KLE cells viability in presence of APS decreased markedly in a dose-and time-dependent manner, and the joint effect of the two drugs had asignificantly higher inhibition rate, showing their synergistic effects. Flow cytometry revealed that TA could cause G1phase arrest. Using APS and cisplatin at the same time can plus the role of promoting cell apoptosis.3. TFA could suppress KLE cell’s proliferation significantly, existing dose dependent. The inhibitory effect was most significant when Joining TFA for24hours.The joint effect of the two drugs had asignificantly higher inhibition rate, showing their synergistic effects(P<0.01). Flow cytometry revealed that TFA could cause G1phase arrest and induce apoptosis at the same time.Conclusion:1. TA can inhibite the proliferation of KLE cells effectively and induced apoptosis, and the two drugs administered in combination show synergistic effects, and showing a dose-and time-dependent manner. This effect may be realized by raising the expression of PDCD5in cells.2. APS can inhibite the proliferation of KLE cells effectively, and this effect shows a dose-and time-dependent manner.3. TFA could suppress KLE cell’s proliferation and induce apoptosis.4. The joint effect of DDP and TA, APS or TFA had a significantly higher inhibition rate,showing their synergistic effects.