| In the last 20 years, the study on reactive oxygen species has become the hot natural topics ofmodern life science. Appraise and selection of resources with strong antioxidative activities havebecome the important trends of biologymedical science,food science and technology.Based on the principle of that a strong sonochemiluminescence signal is generated by ROSwhich are produced in the solution of water by ultrasound, but the reactive oxygen species could beeliminated by the antioxidants or reductant,which quench sonochemiluminescence signal. Themethod can be applied to estimate anti-reactive oxygen species (ROS) capacity of antioxidants orreductant. The method can approach high detection frequency, simplicity and automatic operation.Five chapters are included in this thesis. The first chapter is review, and the rest are researchreports. In the first chapter, a brief review was given on the generation harmness of ROS, sorts ofantioxidant and analytical methods for the determination antioxidation capacity. In the researchreports, main content is as follows:1. Various factors including extracting temperature, extracting time and times of extraction, thataffect on the anti-reactive oxygen species (ROS) capacity of extracted tea from five kinds tea areinvestigated by the flow-sonochemiluminescence method. Experiment results show that the optimumtea infusing condition are: 96? water, 30 minutes infusing time and three times infusing frequency.Tea can effectively restrain the sonochemiluminescence of luminol induced by reactive oxygenspecies on line. Descending order of extracted tea anti-ROS capcity of studied teas is Zi yang maojian> Oolong tea>Shou mei>Keemun black tea>Pu'er tea.2. Various factors including extracting temperature, extracting time and times of extraction, thataffect on the anti-reactive oxygen species (ROS) capacity of extracted tea from different price of teasare investigated by the flow-sonochemiluminescence method. Experiment results show: Thestrongest anti-reactive oxygen species ROS of tea can gain at the following conditions: 96°Cinfusing temperature, 40 minutes infusing time and one times infusing frequency; under differentconditions, relationship between the price and the anti-reactive oxygen capacity is no obvious.3. Based on flow-sonochemiluminescence method,dissolution test of tea, Isoniazd and Vctablets are studied. Experiment results show: The system responds linearly to Isoniazd concentrationin the range of 0.01～1 mg/ml. The system responds logarithm to L-Ascorbic concentration in therange of2.0×10～(-4) mg/ml～1.0×10～(-2) mg/ml. Dissolution testing of substance are described: dissolutionof tea is quick in ten minutes and dissolution is complete at seventeen minutes; dissolution ofisoniazd tablet is relatively quick in ten minutes and dissolution has completed at thirty minutes;isoniazd tablet is relatiVely quick in ten minutes and diSS01utiOll has completed at thirty minutes； dissolution of Vc tablet is very quick in six minutes and dissolution has completed at thirty minutes.Experiment demonstrate that automatic operation and high throughput, in which analysis frequencyis 356 samples h～(1) can be achieved by flow-sonochemiluminescence method. It can determine thedissolution profiles of the tested substances. It can be used as on line dissolution monitoring of thetested substances easily and exhaustivly.4. This research is based on the triphenylmethane class compound as the parent substance.Fluorescence probe synthesized has the selective to the metallic ion. Rhodamine B hydrazine, acolorless and non-fluorescent substance, gave weak fluorescence. When Ru～(3+) was introduced into asolution of Rhodamine B hydrazine, a strong fluorescence and absorption can be detected. Basedon this, the method of determination of Ru～(3+) by flow-injection analysis was primary established.The system responds linearly to Ru～(3+) concentration in the range of 2.0×～10(-6)～1.O×10～(-5) mol/L withcorrelation coefficient of 0.9983 and a detection of 6×10～(-7) mol/L. The relative standard deviation(RSD) was 2.1% for 3.0×10～(-6) mol/L (n=11). |
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