Skullcap Fingerprint And Profile-effect Relationship Study

For Radix Scutellariae,our predecessors have done more in-depth studies,in particular,its main component baicalin studied,2005 edition of Pharmacopoeia use the quality of Baicalin as evaluation indicators of ingredients,and provides its content is not low in 9%.At home and abroad a large number of studies have shown that Scutellaria from different areas of several major components of Baicalin different,and some significant differences.Generally agreed that the best quality production Baicalin Chengde,Hebei.At the same time,in the antipyretic, anti-inflammatory effects,is better than other parts of baicalin,but show that its Baicalin not high than other places.Prompted by baicalin as the evaluation of quality standards is limited.Baicalin is not entirely representative of the level and quality of good or bad baicalin.The pharmacological effects of Scutellariae baicalin not reflect by baicalin,in some role in efficacy,other composition may play a key active role.This study is the second step of subject,the further extraction and separation of Scutellariae,the optimization of building a fingerprint, and other colleagues with the group doing a combination of pharmacological experiments,through the method of gray relation research to study the relationship between spectral efficiency,and give the following findings:1 This chapter contains two parts.In the first part,documents about the research progresses in chemical constituents of Radix Scutellariae past years were summed up and analyzed,In the second part,we made an overview about fingerprint of traditional Chinese medicine.The last part is about the overview of the spectrum-effect relationship2 Theoretical discussion,from the complexity of system to study the relationship between spectrum and effect.3 This experiment done on the extraction and separation of Baicalin,and a number of important flavonoids,including baicalin,wogonoside,baicalin, wogonin,oroxylum-A(oroxylin A),7-methyl chrysin.4 Analyzed different sources of Baicalin samples with HPLC the chromatographic conditions(gradient elution,column) for the further optimization of the completion of a systematic approach to school inspection,building Baicalin fingerprint.Cluster analysis method used to quantify the characteristics of the fingerprint(area) to a computer cluster analysis.In practical applications,the sample can be described by this study,an analysis of chromatographic conditions, quantification of fingerprint characteristics will be entered into the computer calculations,we can quickly arrive at the results of classification.This study has theoretical and practical value.5 The research on Baicalin fingerprint characteristics and the method of spectrum-effect relationship.Baicalin fingerprint in pharmacodynamics quantitative data and,based on gray relational analysis of the use of technology, looking for fingerprints of the characteristics of the chemical composition represented by the contribution of the effect.The results showed that:the antipyretic effect of baicalin is its"group of chemical composition,"the result of the common.Based on the results of correlation to determine the composition of the"fever effect"contributed to the size of the order:Baicalin> The material of retention time of 74.1min> baicalin>wogonin>wogonoside.And the composition of the"anti-influenza virus,"the size of contributions to the following order: Baicalin> The material of retention time of 74.1min>The material of retention time of 58.5min>wogonin> baicalin> wogonoside.6 Summary and discussion...