Weak Vitality The Sperm Akap82mrna Expression And Significance

Objective:AKAP82(A-kinase anchor proteins82) is one of A-kinase anchor proteins in sperm.In order to know the AKAP82mRNA levels of the patients with asthenospermia,we examinated the expressions of AKAP82mRNA in the patients and the healthy fertile men.Methods:Sperm samples were obtained from 59 patients with asthenospermia (experimental group),and the normal sperms were obtained from 28 healthy fertile men(control group).And we estimated motility parameters with a computer-assisted semen analysis(CASA) technique.AsAb(antisperm antibody) of blood serum in the experimental group's was measured by ELISA(enzyme-linked immunosorbentassay). We measured the two groups AKAP82mRNA by reverse transcriptase PCR.At last, after the experimental group was treated,we measured the levels of AKAP82mRNA by reverse transcriptase PCR.Results:Among the 59 patients,15 patients had been found with AKAP82mRNA microdeletion,and among the 28 healthy fertile men,2 had been found with AKAP82mRNA microdeletion,the percentage of g type sperm and the total tail swelling rate were significantly different(P＜0.05) between control group and experimental group.Conclusion:Abnormality of AKAP82 expression in sperm may be one of the etiological factors in asthenospermia.Abnormality of AKAP82mRNA results in the abnormality of AKAP82 expression.