Vascular Cognitive Impairment, The Expression Of Upa In The Blood Of Patients With Cerebral Infarction And Genotype Analysis

Objective: To measure the urokinase-type plasminogen activator in plasma of patients with cerebral farction and Vascular Cognitive Impaiment,and to study the effect of uPA on cerebral in farction and Vascular Cognitive Impaiment .Methods: ELISA sandwich method was used to measure the plasma levels of uPA in 95 patiens with Vascular Cognitive Impaiment and subdivided into mild, moderated and severe subgroups, 114 patients of cerebral farction and the 100 healthy persons as control.Results:1 Table1 showed the three groups of uPA content.2 Table.2 showed the VCI patients in different condition of uPA content.3 Vascular Cognitive Impaiment mild, moderated and severe groups, the uPA levels in plasma are(1.313±4.40 )ng/ml,(1.316±3.94)ng/ml,(1.731±6.82)ng/ml respectively, and the levels of uPA in the plasma of patiens with cerebral farction is (1.573±4.480)ng/ml,which are significantly higher than than those of healthy persons(0.953±3.36)ng/ml,(p<0.01).The levele uPA in mild Vascular Cognitive Impaiment is lower than that of uPA in the both moderted and severe groups, (p<0.05). The level uPA in the patients with Vascular Cognitive Impaiment is not different significantly from those of the cerebral farction groups. Conclusions1 The plasma levels of uPA increase in patients with Vascular Cognitive Impaiment and cerebral farction, but there is no difference between of them.2 Fortheremore, the levels of uPA are related with the severity of Vascular Cognitive Impaiment. Objective: Vascular Cognitive Impaiment is cognitive impairment which is caused by ischemia or hemorrhagic cerebrovascular diseases and global cerebral ischemia or hypoxia.VCI patients have various impairments of language, sports , visual spatial and personality obstacles especially in defects of memory and cognitive function. According to epidemiological investigation in our country VCI account 60% of senile dementia. For the declining of living ability, it threats the elderly health and quality of life seriously , which bring a huge burden to family and society , so the study to VCI in various aspects gets attention more then over. The risk factors to VCI mainly including hypertension, hyperlipidemia, hyperhomocysteinemia and diabete such factors connect with small vessel disease. Bad living habits connecting with VCI include lack of exercise, unhealthy diet , smoking, drinking and drug abusing. Besides, age and level of education are also of the risk factors. In recent years , genetic research to VCI risk factors show that a variety of environmental factors and multiple genes make VCI. Currently , there are many reports about AD and cerebral strokes'related genes but there is so little report about VCI. So in order to research VCI related genes we could reference the results about AD and cerebral stroke. These related genes polymorphism may connect with VCI. Recent studies show that plasminogen system partipciates AD patients brain's cleaand degradation. Among this, urokinase-type plrance asminogen ac-tivator which is coded by gene PLAU play an important role in the clearance of Aβ. The aim of this study is to investigate the association of uPA gene PLAU with Vascular Cognitive Impaiment.Methods: The uPA gene PLAU polymorphism were analyzed by PCR-restriction fragment length polymorphism in 39 VCI patients ,28 NDCI patients and 23 unrelated control individuals. Determination serum triglyceride levels, analysis of high blood pressure, diabetes, high cholesterol, smoking, PLAU gene polymorphism and body mass index point correlation with the incidence of VCI.Results:1 Genda,age,BMI,level of education showed no significantly difference between any two groups. The hypertension showed significantly difference between VCI group and NC group(p<0.05); case history of stroke showed significantly difference between VCI group and NDCI group(p<0.05).2 The frequencies of the rs2227564 C and T allele among VCI patients and normal controls were 60.0%, 40.0% and 63.5%, 36.5%, respectively; No significant difference in the rs2227564 allele distribution was shown between VCI patients and normal controls (P>0.05). The distribution of the C/C, C/T and T/T genotypes between endometriosis patients (35.8%, 48.4% and 15.8%, respectively) and normal controls (35%, 57% and 8%, respectively) also had no significant difference (P>0.05). Compared with the C/T+T/T genotypes, the C/C genotype could not increase the risk of developing VCI, the odds ratio was 1.04(95%CI=0.58~1.86).3 The frequencies of the rs2227564 C and T allele among NDCI patients and normal controls were 59.6%, 40.4% and 63.5%, 36.5%,The frequencies of the C/C, C/T and T/T genotypes among NDCI patients (34.2%, 50.9% and 14.9%, respectively) were significantly different from those in normal controls (35.0%, 57.0% and 8.0%, respectively) (P<0.05). Compared with the C/T+T/T genotypes, the C/C genotype could significantly increase the risk of developing NDCI, the odds ratio was 0.97 (95%CI=0.55~1.70).4 The frequencies of the rs2227562 A and G allele among VCI patients and normal controls were 30.0%, 70.0% and 32.5%, 67.5%, respectively; No significant difference in the rs2227562 allele distribution was shown between VCI patients and controls (P>0.05). The distribution of the A/A, A/G and G/G genotypes between VCI patients (9.5%, 41.0% and 49.5%, respectively) and controls (14%, 37% and 49%, respectively) also had no significant difference (P>0.05). Compared with the A/A+A/G genotypes, the G/G genotype could not increase the risk of developing VCI, the odds ratio was 1.02(95%CI=0.58~1.79).5 The frequencies of the rs2227562 A and G allele among NDCI patients and normal controls were 48.7%, 51.3% and 51.0%, 49.0%, respectively; No significant difference in the rs2227562 allele distribution was shown between NDCI patients and controls (P>0.05). The distribution of the A/A, A/G and G/G genotypes between VCI patients (9.6%, 39.5.0% and 50.9%, respectively) and controls (14%, 37% and 49%, respectively) also had no significant difference (P>0.05). Compared with the A/A+A/G genotypes, the G/G genotype could not increase the risk of developing NDCI, the odds ratio was 1.10(95%CI=0.64~1.88).Conclusions:1 Case history of stroke and hypertension showed significantly difference between VCI group and control groups. Stroke and hypertension may be risk factors to vascular cognitive impairment.2 PLAU gene Rs2227564 C/T genotype and C allele showed higher distribution ,but no significant differences between any two groups,which suggested that PLAU gene Rs2227564 polymorphisims might not be hereditary factors contributing to VCI.3 The proportion of A allele and the frequency of G in VCI group showed no significantly difference between NDCI group and NC group. It showed that PLAU gene rs2227652 polymorphism had no collection with the risk of developing VCI.4 PLAU gene rs2227564 and rs2227563 polymorphism had no collection with the risk of developing NDCI.