The. Curcumol Induced Experimental Study Of Human Gastric Cancer Bgc-823 Cell Apoptosis And Ros Relations

Apoptosis are referring to cells in certain physiological or pathological conditions, to follow its own procedures, gene regulation by the death of the initiative process. From the new view angle about apoptosis to view the effect of Chinese crude drug opposing tumor, to reveal mechanism of action of Chinese crude drug, screening new anticancer Chinese crude drug possesses full crucial significance. Curcuma are Zingiberaceae rhizomes of perennial herbaceous plants.Chinese medicine often Curcuma with Trigonobalanus, Salviaatractylodes, Compatible applications, such as the treatment of various tumors. Curcumol, also known as turmeric, Central Austria alcohol, are volatile oil from Curcuma extract effective monomer composition. Curcumol antibacterial, antiviral activity in clinical practice has been widely applied, but its role in anti-tumor research at home and abroad is still less. The subject of curcumol antitumor effect and mechanism study sought to curcumol become an antitumor drugs.1. Objective:By detecting curcumol on cultured human gastric cancer BGC-823 cells proliferation was observed morphological changes of apoptosis, detection of apoptosis rates, and intracellular reactive oxygen species (ROS) level changes. Discussion curcumol induced human gastric cancer BGC-823 cells apoptosis mechanism curcumol further elucidate the role of antitumor mechanism for curcumol antitumor effect provide a basis for clinical application.2. Methods:Will curcumol in ethanol solution. Experimental Group A,B,C,D group, for the prorata allocation of the concentrations were 12.5,25,50,100μg / mL of culture medium curcumol. Negative control group containing 1% ethanol medium.MTT deoxidized method was used to detect different concentrations of curcumol on human gastric carcinoma BGC-823 cells proliferation. AO/EB ambi-fluorescent and Fluorescence microscope after different concentrations of curcumol role, the human gastric cancer BGC-823 cells in the morphological changes of apoptotic cells.FITC-Annexin V and PI ambi-staining method, detected by flow cytometry after different concentrations of curcumol role, the human gastric cancer BGC-823 cells apoptosis rate. By flow cytometry, fluorescent-labeled by indirect method through different concentrations of curcumol role, the human gastric cancer cells BGC-823 changes in the level of reactive oxygen species.3. Results:3.1 MTT assay at different concentrations curcumol treatment of human gastric cancer BGC-823 cells 24,48,72 hours, can inhibit cell proliferation. Dose, time-dependent, add drug group and control group inhibition rates were significantly different(P<0.05).3.2 Observed that the EB fluorescence microscope for the red, only the death of nuclear staining. Normal living cell membrane integrity, EB should not infiltrate the nucleus should not stain. A0 for the green, can be used to staining of living cells. Control group shows that the whole cell is green indicating that the need to normal living cells. After curcumol role, the entire cell background for the green, a more complete picture of the cell membrane, nucleus of red and green overlap for orange nuclei showed pyknosis or bead-like shape, that is, when the morphological changes of apoptosis (apoptotic body).3.3 According to mapping by flow cytometry, FITC+/PI- to apoptotic cells. Human gastric cancer BGC-823 cells after different concentrations of curcumol role, the proportion of apoptotic cells increased significantly, and dose, time-dependent. 12.5, 25,50,100μg / mL curcumol role 24,48 hours, add drug group the rate of gastric cancer cell apoptosis compared with the control group, there was a significant difference(P<0.05).3.4 Immunofluorescence using flow cytometry the level of intracellular reactive oxygen species. The results showed that different concentrations after curcumol role in human gastric cancer BGC-823 cells, intracellular reactive oxygen species levels and curcumol increased dose-dependent manner.12.5,25,50,100μg/mL Curcumol role 24,48hours, add drug group compared with the control group, there was a significant difference(P<0.05).4. Conclusion:4.1 Concentration 12.5,25,50,100μg / mL inhibited curcumol human gastric cancer BGC-823 cells proliferation.4.2 Concentration 12.5,25,50,100μg / mL curcumol can induce human gastric cancer BGC-823 cells apoptosis.4.3 Curcumol induced human gastric cancer BGC-823 cells apoptosis with an increase in the ROS generated.