| BackgroudCerebral infarction is a emergent disease endangering the people'life and work because of high disability and mortality. are, stroke prevention and treatment become important topics of social concern. To save the ischemic penumbra is the key to treatment of acute cerebral infarction.To induce surrounding tissue ischemia angiogenesis and collateral blood vessel formation, and reduce neuronal death are the key to treatment. Past research has shown that stroke patients with brain infarction and penumbra (in case penumbra) microvessel density was significantly higher than normal brain tissue. And prove that the surrounding area infarction microvascular density is positive correlation wirh prognosis of stroke in patients, and HGF participate in the protection of cerebral ischemia and take a role of angiogenesis,etc, which is important for neuron survival after stroke. Thus, HGF and angiogenesis become a popular treatment of cerebral infarction.Methods72 male Wistar rats were randomly divided into sham operation group and the ischemic group. Cerebral ischemia models of rats were established by blocking the middle cerebral artery. The expression of HGF and VEGF in the two groups were detected by immunohistochemical method on different times of 2h,6h,12h,24h,3d,7d,14d,20d. Brain edema was assayed by using dry-wet weight method,and the use of immunohistochemical method is to observe the brain tissue HGF and CD31 expression. Using SPSS11.0 statistical software to proceed the data analysis.ObjectiveTo study the Expression of hepatocyte growth factor (HGF) and CD31 in cerebral ischemic rat and their relationship. To investigate the pathological mechanism of cerebral ischemia.ResultHGF in the sham-operated group is almost without expression, the expression of HGF increased after ischemic 2h, and gradually increase with time in expre ssion, ischemia group(6h,12h,24h,3d,7d,14d,20d)compared with the control group had significant difference (P <0.01).Microvessel density (CD31) expression at 2 hours decreased ,at 24h after the expression started to increase, at 3d-7d the expression of CD31 was significantly enhanced when the peak.At14d-20d,the expression of Microvessel density decreased. ischemia group(12h,24h,3d,7d,14d,20d)compared with the control group had significant difference (P <0.01).ConclusionThe expression of HGF and microvessel density increased in cerebral ischemia , and all the ischemic changes in the expression of timing characteristics are basically the same, are closely related. HGF may contribute to microvascular reconstruction and the expansion,both the expression of HGF and microvessel density in the cerebral ischemic injury play an important role in repair of cerebral ischemia. |
PDF Full Text Request