Kgf In The Mechanism Of Angiogenesis In Rat Corneal Alkali Burn

Objective:To observe the expression of Keratinocyte Growth Factor(KGF) in the normal and alkali-burned corneas of rat.Methods:Thirty-five Wistar rats were randomly divided into control group(five eyes) and experimental group(thirty eyes).In experimental group,we burned eyes by 1mol/L NaOH to establish the alkali-burned model,while in control group,we replace 1mol/L NaOH by PBS.According to the time after alkali-burned,we further divided the experimental group into six groups,including six-hour group,one-day group,three-day group,five-day group,seven-day group and fourteen-day group.The cornea structure at different stages after alkali burned is observed by light microscopy after HE staining.And KGF was detected in rat normal cornea and in that after alkali burned by immunohistochemistry at different time.Results:Immunohistochemistry method showed that KGF was not obviously observed at normal rat corneas. After alkali burned,the expression of KGF increased immediately,and peaked at 7 days after cautery,then decreased,and significant decreased to near base line after 14 days.The expressions of KGF correlates with cautery induced angiogenesis in the rat cornea.Conclusions:KGF perhaps plays a very important role in CNV induced by alkali burn.Inflammatory cells perhaps contribute to this process.