Hydrogen Sulfide On The Ischemia-reperfusion Rat Heart And Its Possible Mechanism Discussed

Hydrogen sulfide(H₂S),as an endogenous gas signaling molecule with important biological function that has been found recently,may play a protection in ischemic reperfusion(I/R) myocardium.We investigated the cardioprotective effect of H₂S in rats model of ischemic reperfusion in vivo and its possible mechanism. Animals were randomly divided into 3 groups and received either vehicle,Sodium Hydrosulfide(NaHS) or DL-propargylglycine(PAG) respectively everyday for 1 week before surgery and the treatment continued for a further 2 days after I/R till the animals were sacrificed.We investigated the plasma H₂S concentration and blood pressure,with the electrocardiogram(ECG) together,to prove the effect of H₂S to the heart function.We also compared the heart infarct size and the expression of an anti-apoptosis gene,survivin,among groups.As the data shown,the NaHS group had great improvement in blood pressur and electrocardiogram situation.And the remarkable shrink of the infarct size and up-regulation of survivin in NaHS group comparing with the other two groups also showed the cardio protective effect of H₂S in our study.Besides,we doubt the protective effect of H₂S may relate to angiogenesis,the result also confirmed that.The conditional media of rat vessel smooth muscle cells(VSMC) with treatment of NaHS can improve the proliferation of rat brain vessel endothelial cells(BCEC).Our study showed H₂S can enhance the stability of Hypoxia Inducible Factor-1α(HIF-1α),which is a transcription factor of vessel endothelial growth factor(VEGF),thereby up-regulated the expression of VEGF.In conclusion,H₂S has shown its protective effect to ischemic rats or cells in our study by anti-apoptosis or advancing angiogenesis.The clear mechanism still wait to be illuminated by next study.