The Effects Of Hydrogen Sulfide (H2S) On Myocardial Ischemic-reperfusion Injury In Rats

Objective:To explore the effect of Hydrogen sulfide (H2S) on apoptosis in myocardia of rats after ischemia-reperfusion injury.Methods:Ischemia-reperfusion model of rat’s heart was established by ligating the left anterior desending coronary arteries (LAD) for30min and unclamped for60min after opening chest. The level of cardiac Troponin I (cTn I) before and after ischemia-reperfusion was measured by applying ELISA; the apoptosis of cardiomyocytes was measured by TUNEL; the level of caspase-3was detected by immunohistochemistry. Hemodynamic indexes of all the rats were determined and their cardiac ischemic and infarction areas were measured.Results:After H2S intervention, the level of cTn I was decreased significantly, and cardiac ischemic and infarction areas were decreased as compared with those of the control group. The expression of caspase-3was reduced by H2S intervention during ischemia-reperfusion injury, therefore reduced the apoptosis of cardiomyocytes.Conclusion:H2S has protective effects on ischemia-reperfusion injury in rat’s heart, and its possible mechanism is through reducing cardiomyocyte apoptosis after ischemia-reperfusion.