| Purpose: To study furostanol saponins from the fruits of Tribulus terrestris L. and select terrestrosin K (â…¢) as the reference substance to establish the HPLC determination method of its content for the quality control of Tribulus terrestris L..Method: By the modern chromatography techniques (silica gel, macroporous resin, Sephadex LH-20, ODS and preparative HPLC, etc), furostanol saponins were isolated from the fruits of Tribulus terrestris L.. The structures of these compounds were elucidated by the spectroscopic analysis (1H NMR, 13C NMR, ESIMS, HMQC and HMBC) and chemical methods. High performance liquid chromatography (HPLC) was used to determinate the content of terrestrosin K (â…¢).Result: Five furostanol saponins were isolated from the fruits of Tribulus terrestris L. Four compounds of them were identificated as: 26-O-β-D-glucopyranosyl-(25R)-5α-furostan -20(22)-en-12-one-2α,3β,26-triol-3-O-β-D-glucopyranosyl(1â†'4)-β-D-galactopyranoside(â… );26-O-β-D-glucopyranosyl-(25R)-5α-furostan-20(22)–en-12-one-3β,26-diol-3-O-β-D-galacto- pyranosyl-(1â†'2)-β-D-glucopyranosyl(1â†'4)-β-D-galactopyranoside(â…¡);26-O-β-D-glucopyr-anosyl-(25S)-5α-furostan-20(22)-en-12–one-3β-26-diol-3-O-β-D-galactopyranosyl(1â†'2)-β- D-glucopyranosyl(1â†'4)-β-D-galactopyranoside(â…¢);26-O-β-D-glucopyranosyl-(25R)-5α-fur- ostan-20(22)-en-2α,3β,26-triol-3-O-β-D-glucopyranosyl(1â†'2)-β-D-glucopyranosyl(1â†'4)-β- D-galactopyranoside (â…£). Terrestrosin K (â…¢) with high content and purity (ï¹¥98﹪) was selected as the reference substance.The methodology investigation showed that the linearity was obtained over the range of 1.1～5.5μg (r = 0.9991), the average recovery was 95.76 % (n = 6), and RSD = 0.50 %.Conclusion: Compoundâ… is a new compound. Terrestrosin K (â…¢) was selected as the reference substance. The HPLC method of its content determination was established. The method is accurate and reliable, and has a high sensitivity and good reproducibility. |
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