Evodia The Angelica Drug Compatibility Studies

Purpose:Win by the soup from the "Jin Gui Yao Lue·Fu Ren Za Bing Mai Zheng Bing Zhi" woman chief "benefit under the disease more than a few on the 10th,the morning that is, fever,and less heating in acute Fu Man,Fan Re the palm of your hand.Lips dry mouth Blood knot......do not lean in less".Side from the original composition of 12 herbs,two of the topics selected and Evodia rutaecarpa and Angelica Sinensis(Oliv.) Diels(a Primary and a secondary) study seeks to clarify the principles of their combination,in order to provide scientific basis for the development of new drugs.Material and method:Material:Evodia rutaecarpa and Angelica Sinensis(Oliv.) Diels were purchased in in the Hebei Anguo;AR2140 electronic analytical balance;AS3120A ultrasonic extractor; U3010-type UV-Vis Spectrophotometer;Agilent1100 United States-based liquid chromatography system,METTLER AB 135-S balance 1/100000;liquid chromatography with acetonitrile to pure water for the re-distilled water;all other reagents is analysis pure.Methods:The drug in the chemical composition of the content and the corresponding pharmacological test as an index to determine the rutaecarpa,Angelica preparation method and the best combined ratio;using L9(34) orthogonal design,selected rutaecarpa,Angelica medicine to the most best extraction technology;the use of thin layer chromatography and high performance liquid chromatography of extracts of drug quality standards for research; the use of high-performance liquid chromatography of drugs for fingerprints on the map.Results:1.Through chemical and pharmacological experiments on laboratory test results,the last two drug combination for the preparation of ethanolic fried together.2.Through chemical experiments and the results of pharmacological experiments,the last election the ratio of the two drugs combined 3:2(rutaecarpa:Angelica).3.Through L₉(3⁴) orthogonal test to determine the drug Danggui rutaecarpa of(3:2) combined the best extraction process was 70%ethanol extract twice 8 times the amount of extract 2h. 4.By measuring 3 samples approved evodiamine,rutaecarpine and ferulic acid,tentatively scheduled for the goods in order to per gram rutaecarpa evodiamine,rutaecarpine dollars, respectively,shall not be less than 2.0 mg·g^-1,2.0 mg·g^-1,per gram of ferulic acid in Angelica, shall not be less than 0.90 mg·g^-1.5.The proportion of different combinations of drugs on the high-performance liquid extract fingerprint line drawing,select the two drugs in a representative composition of the two indicators,with their ratio of peak height ratio to determine the compatibility of their quality provide the basis for stability.Conclusion:The subject of Evodia rutaecarpa Angelica Sinensis(Oliv.) Diels of warm soup by two extraction methods had a taste,the best combined ratio and the choice of extraction and tentative quality standards and its fingerprint drawing,method stability and feasible for a small Chinese medicine Angelica Rutaecarpa development and application of compound to lay a solid foundation.