Extraction Of Curcuminoids Isolation And Identification Of The Chemotherapy Drugs Synergy Of Anti-tumor Effect

Objective: To extract and isolate curcuminoids from Curcuma longa and study the curcumin alone combinated with antineoplastic agent in human fibrosarcoma HT1080 cell,human breastcancer MCF-7 cell,human lung cancer adenocarcinoma A549 cell on the proliferation inhibitory effects and apoptosis-induced synergistic effects, which in order to explore its synergistic anti-tumor mechanisms, the results might be provided an experimental basis and theoretical foundation for effective chemotherapy synergist.Method: The 80% EtOH extract was subjected to silica gel column chromatography. Chemical structures were identified by physico-chemical properties and spectral analysis; The effects of curcuminoids on proliferation inhibition of HT1080 cell line, MCF-7 cell line, A549 cell line were detected by MTT assay; The effects of curcumin in combination with cisplatin, adriamycin, hydroxycamptothecin on proliferation inhibition of HT1080 cell line, MCF-7 cell line, A549 cell line were detected by MTT assay; The morphological analysis of A549 cell apoptosis were detected by Hoechst 33258 staining; Bcl-2 protein expression in A549 cell were detected by Western blotting.Result:(1) Three curcuminoids were isolated from Curcuma longa and identified as curcumin(Ⅰ), demethoxycurcumin (Ⅱ), bisdemethoxycurcumin (Ⅲ);(2) Curcumin, demethoxycurcumin, bisdemethoxycurcumin all showed significantly proliferation inhibition on HT1080 cell line, MCF-7 cell line, A549 cell line in vitro, the curcumin indicated the best proliferation inhibition activity, the IC50 were respectively 10.39μmol/L,11.85μmol/L and 10.25μmol/L.(3) In a certain concentration range, the curcumin in combination with cisplatin, adriamycin, hydroxycamptothecin on HT1080 cell line, MCF-7 cell line, A549 cell line has synergistic or additive effect on proliferation inhibition.(4) Curcumin combinated with adriamycin has synergistic effcct on A549 cell apoptosis, its mechanisms may be through down-regulating Bcl-2 protein expression to the implementation.Conclusion:Curcumin in combination with cisplatin, adriamycin, hydroxycamptothecin on HT1080 cell line, MCF-7 cell line, A549 cell line has synergistic or additive effect on proliferation inhibition, the synergistic anti-tumor mechanism may be related to apoptosis-induced and down-regulating Bcl-2 protein expression. The results have shown that curcumin combined with chemotherapy drugs for the treatment of some tumor may be have the possibility, which have synergistic effect in proliferation inhibition of human tumor cells, it might be provided an experimental basis and theoretical foundation for effective chemotherapy synergist.