Summer Experimental Study On Rats With Cerebral Infarction Of Nos, Expression Of Inos And Of Nscs, Syn Expression

Objective:Detecting the expression of nitric oxide synthase(NOS),inducible nitric oxide synthase (iNOS) in blood serum of rats and the pathological of the hippocampus by hematoxylin and eosin (HE) and content of neural stem cells(NSCs),synaptophysin (SYN) in the hippocampus tissue of rats that corydalis decumbens improved the situation of middle cerebral artery occlusion(MCAO) model in rats. Exploring the effects that corydalis decumbens whether could contribute to the Brain Plasticity in rats. Provide the experimental basis for corydalis decumbens clinically.Methods:(1)Model:cerebral ischemic(CI) model was formed by adopting the MCAO method using intralumina thread in health male Whister rats. (2)Groop:According to the literature using random number table this model success were randomly divided into four groups:model group, high dose of Corydalis decumbens group, low dose of Corydalis decumbens group group, and Hydroxyethylrutin group ; setting up a blank group and sham model group. (3) Delivery methods:For 7 days, blank group,sham model group and group model group were intragastrically given to rats by a dose of 6ml/kg/d physiological saline. While the rats in low dose of Corydalis decumbens group, high dose of Corydalis decumbens group group, and Hydroxyethylrutin group were intragastically given different medicine:to. 2.4g/kg/d,9.6g/kg/d Corydalis decumbens and 144mg/kg/d Hydroxyethylrutin.. (4)5—bromodeoxyuridine(BrdU) labeled:Every groups rats was injected intraperitoneally BrdU physiological saline (30mg/kg/d) 2 times at 8—hour intervals on day 7. (5)Detect indicators: Measured blood serum NOS,iNOS absorbance of NOS,iNOS activity was by spectrophotometry. HE staining of pathological changes of the hippocampus. 5—bromodeoxyuridine labeling protocols and immunohistochemistry detection of rat hippocampal BrdU and SYN protein expression was observed.Results:(1) HE pathological of changes:blank group and sham model group had no obvious pathologica changes; Model group has loose framework, never cells cytomorphosis, karyopycnosis; Three therapeutics groups has a bulk of normal morphous and formation of never cells,small cells cytomorphosis and karyopycnosis. (2) NOS and iNOS detected:blank group and sham model group can reduce the activity of NOS and iNOS compared with the model group(P <0.05); treatment groups can reduce the activity of NOS and iNOS compared with mode group(P< 0.05), but no difference between the three treatment groups significantly (P>0.05). (3) About the Brain Plasticity detected:NSCså'ŒSYN. blank group and sham model group had rarely NSCså'ŒSYN expression, Another groups more NSCså'ŒSYN differences expression(P< 0.05).Three reatment groups had more significant rises in NSCså'ŒSYN expression (P<0.05). but no difference among treatment group significantly (P>0.05)Conclusions:(1) MCAO method using intralumina thread had well operation and stability.It was a perfectly model for CI model.(2) Corydalis decumbens improved the pathological change of the hippocampus of the CI model. Corydalis decumbens can prevent and treatment CI model. (3) Corydalis decumbens can reduce the activity of NOS and iNOS, to decrease NO.It can be see that Corydalis decumbens can lesse neurotoxicity by NO, blocking cascade reaction to protect nevers cytoprotection. It was problely that Corydalis decumbens should enhance the cerebral plasticity. (4) Corydalis decumbens can increase the NSCs and hold-back the declined expression of SYN in hippocampus. It could promote the synaptic re-establishment and connection. Corydalis decumbens may contribute to the cerebral plasticity. (5) Corydalis as well as Hydroxyethylrutin can contribute to the cerebral ischemic. It'cure independency of dose.