Notoginseng Compound Granules Pharmacodynamic Studies And Mechanism Of Inhibition Of Thrombosis

Purpose:To observe the pharmacodynamics and mechanism of Radix Notoginseng compound to inhibition thrombosis.Material and method:To observe Radix Notoginseng compound on electrical stimulation-induced arterial thrombosis model for thrombus formation time, thrombus length, hemorheology and thrombosis patterns. Measured by radioimmunoassay Radix Notoginseng compound of the adrenaline and cold-induced blood stasis model of the impact of TXA₂ and PGI₂ levels. Determination of Radix Notoginseng compound of the main factors and important factors.Results:1. Electrical stimulation-induced arterial thrombosis model: Radix Notoginseng compound each dose as well as the aspirin group was significantly longer able to due to electrical stimulation of the formation time of arterial thrombosis (P <0.05), in which aspirin group of the most significantly (P<0.01); could significantly shorten the length of thrombosis (P<0.05). Radix Notoginseng compound each dose group as well as the aspirin group can significantly lower hematocrit and red blood cell deformability index (P <0.05), of which Radix Notoginseng compound effects of high-dose group of the most significant (P <0.01); with the model group comparison, the aspirin group can significantly reduce the erythrocyte electrophoresis time, whole blood viscosity and Casson yield stress (P<0.05), Radix Notoginseng compound of various doses significantly reduced cell gel electrophoresis time and whole blood viscosity (P <0.01); compared with model group, aspirin group and Radix Notoginseng compound group had no significant effect on the Casson viscosity. In the orthogonal experiment, the factor Radix Notoginseng F = 11.322, (P <0.01).2. Hanning blood stasis model: Compound Danshen group could significantly increase plasma 6-Keto-PGFlαand 6-Keto-PGFlα/ TXB₂ content (P<0.01), pairs of TXB₂ had no significant impact; with the model group, Radix Notoginseng compound 2.4 crude drug g /kg group significantly increased plasma 6-Keto-PGFlαcontent (P <0.01) Radix Notoginseng compound 1.2crude drug g /kg group could increase plasma 6-Keto-PGFlαcontent (P<0.05), Radix Notoginseng compound in each group can significantly increased 6-Keto-PGFlα/ TXB₂ values (P <0.01), significantly reduced TXB₂ levels (P<0.05).Conclusion:1. Electrical stimulation-induced arterial thrombosis model: Radix Notoginseng compound can improve the blood rheology and delaying the formation of thrombus. Radix Notoginseng is the main factors for this compound, amber and ginseng are important factor, considering all the drug should be: Radix Notoginseng 2.4 crude drug g /kg, amber 2.4 crude drug g /kg, ginseng 1.2 crude drug g /kg.2. Hanning blood stasis model: Radix Notoginseng compound may be by changing the metabolic pathway of arachidonic acid, inhibit platelet synthesis and the release of TXA₂, is to promote the synthesis of vascular endothelial cells and the release of prostaglandin PGI₂, so that the blood TXA₂ and PGI₂ in concentration in a dynamic equilibrium state, so as to achieve the antithrombotic effect.