| Objective:To investigate the morphological changes of neovascularization after the animal model of oxygen-induced retinopathy (OIR) using different dosage RuboxistaurinMethods:7-day-old C57BL/6J mice were divided into normal control group, hyperxia group, medical treated group and control group randomly. Except for the normal control group, other groups were exposed to 75% oxygen for 5 days and then to room air. As soon as took them into air, the treated group was given with different dosage Ruboxistaurin to its stomach, the control group given different dosage mesylate as well. Then all the mice were sacrificed at postnatal day 17. The proliferative neovascular response was estimated by observing the vascular pattern through fluorescence microscopy and counts of nuclei of new vessels extending from the retina into the vitreous.Results neovascularization and avascular retina were seen in the hyperxia group. The numbers of new vascular cell nuclei extending into the internal limiting membrane and neovascularization were less in Ruboxistaurin group than hyperxia group and control group, showing a significant difference between them (P< 0.01).Conclusion:the animal model of oxygen-induced retinopathy was successful. The neovascularization and avascular retina was more than that of normal group. Ruboxistaurin could decrease the neovascularization and avascular in the OIR. |
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