Dropping Pills Pharmaceutical Research Bitter Stilbene

Purpose: In this paper, basic on the theories of Chinese medicine, combined with advanced modern science and technology to study a comprehensive standardized research on the compatibility of Kuqi dropping pill.Material and method: Astragalus selected by orthogonal design, matrine and drugs on the best extraction method; use of parallel single-factor experiments and orthogonal design method to determine pain Stilbene Pills molding process; using visible - Determination of bitter pills of bitter stilbene ginseng total alkaloid content; the use of high-performance liquid chromatography, on the bitter pills of the active ingredient Qi qualitative and quantitative as well as pharmacokinetic studies; using immunological methods bitter Stilbene Pills efficacy study.Results:1. According to the pre-test to determine the astragalus, matrine drugs on the best preparation method for the alcohol extract co-frying method, the best compatibility ratio of 1:2.2. Using orthogonal design method to determine the astragalus, matrine drugs on the optimal extraction process as follows: Astragalus, matrine the drug (dose ratio of 1:2 according to their hygiene compatibility), to 18 times the amount of 60% ethanol extract three times, Every time 1 hour.3. Selection of single factor experiments and orthogonal design parallel to determine the bitter pills of the molding process stilbene as: PEG6000: PEG4000 = 3:2 as the substrate, drug ratio of 1:2 with the matrix, liquid paraffin (upper): 2 Methyl silicone oil (lower) = 1:3 as a cooling agent, liquid temperature of 80℃, with 20 to 25 drops per minute, the speed, trickle-down of around 10℃cooling agent, the drop from 5 to 8 cm, dropping Pill appearance of the best quality.4. Stilbene using TLC method bitter pills in oxysophocarpine, sophoridine, oxymatrine and Astragaloside for identification, spots were clear separation, Rf values, methods The repeatability and specificity are good.5. Using ultraviolet spectrophotometry and high performance liquid chromatography method bitter pills in the Qi moving indicator element determination, good linearity, precision, stability, reproducibility and recoveries were up to standard.6. By detecting the level of serum hemolysin in mice, macrophage phagocytosis, immune organ weight situation and serum interleukin-2 (IL-2) levels of four indicators showed that bitter pills Gao Qi, middle and low-dose group, positive control group and the control group had significant differences, and showed a definite dose-effect relationship.Conclusion: Through a comprehensive standardization of bitter Stilbene Pills study to determine the compatibility of the optimal ratio of drug preparation, extraction methods and molding technology, the establishment of a bitter Stilbene Pills quality standard system, further improved the pain pills Pharmacodynamics of stilbene and pharmacokinetic study. Developed a stronger efficacy, active ingredients and mechanism of a clear, quality control, product with high technological content Pill agent, has broad application prospects, and Chinese medicine as further research and development to provide reference.