Uncultured-dependent Approach On Bacterial Diversity In Yunnan Non-ferrous Metals Mining

Bioleaching technology not only can deal with low-grade ore and refractory tailings effectively, but also can reduce the pollution of environment, and even some microorganisms in mining areas can remedy the polluted environment. Yunnan province is known as non-ferrous metals kingdom in china. However, the systematic study in bacterial diversity of non-ferrous metal mines in Yunnan province has not been reported. In order to make the best use of microorganisms in mineral industry, related research was carried out at the laboratory.Samples were collected in three typical non-ferrous metal mines in Yunnan province. In this study, we extracted total genomic of samples and built the bacterial16S rRNA hypervariable region gene library to analyze bacterial diversity of these mining areas. Library sequencing results showed that the bacterial species in mine samples are abundant. The gene library of Lanping lead-zinc Deposit contains120clones. They belong to4groups:Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria. These4groups can be further subdivided into27genera:Enhydrobacter, Elizabethkingia, Propionibacterium etc. Dominant flora in this gene library is Proteobacteria. It contains62clones and accounting for51.7%of the total number of clones. The second is Firmicutes. It contains31clones and accounts for25.8%of the total number of clones. The gene library of Beiya Gold Deposit contains120clones. They belong to4groups:Bacteroidetes, Proteobacteria, Actinobacteria and Firmicutes. These4groups can be subdivided into23genera: Hymenobacter, Serratia, Moraxella etc. Proteobacteria is the dominant flora in this gene library. It contains82clones and accounts for68.3%of the total number of clones. The second is Bacteroidetes. It contains15clones and accounting for12.5%of the total number of clones. The gene library of Wenshan bauxite contains120clones. They belong to5groups:Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria and Fusobacteria. These5groups can be subdivided into25genera:Aeromonas, Pseudomonas, Shigella et al. Dominant flora in this gene library is Proteobacteria. It contains83clones and accounts for 69.2%of the total number of clones. The second is Firmicutes. It contains24clones and accounts for20%of the total number of clones.Six samples collected in Wenshan bauxite were analyzed with flow cytometry (FCM). It showed that all the samples can find the second largest dominant flora in dot plot except the3rd sample. Images of dot plot indirectly prove that bacterial diversity in Wenshan bauxite's sample is abundant and also shows the approximate distribution of bacterial communities in samples. At the same time, the bacterial diversity of three mining areas was analyzed with metagenome. The results are still under process.In short, the bacterial species in these typical non-ferrous metal mines are abundant and is mainly Proteobacteria. Every mining area has its own unique bacterial communities, and also has the same genera. Some of these microorganisms have high similarity with uncultured bacteria, and it indicates that new taxa may exist in these typical non-ferrous metal mines.