Isolation And Characterization Of Microsatellite Loci In The Tibetan Fox (Vulpes Ferrilata)

Tibetan fox (Vulpes ferrilata) is widely distributed in the Qinghai-Tibet Platean of China, and it is the important components of plateau ecosystem, what's more, Tibetan fox is the definitive host of hydatid anthropozoonosis. Based on these, it has a practical significance for the highland ecosystemstability and analysis on the transmission route of echinococcosis to research the Tibetan fox's population dynamics and the role do them play in the mechanism of echinococcosis transmission route. It's feasibility to conduct the research on the population structure of Tibetan fox with the help of molecular biology technique, which breaks the limitation of macroecology application. With the rapid development of experimental technique of molecular biology, microsatellite marker plays a significant part in the population genetics concerning multispecies and ecology field. As a codominant molecular marker, microsatellite technique will have a more comprehensive application prospect with the advantages of widely spreading in eucaryon, rich contents about polymorphism information, be in common use between allied species and low loss of DNA samples.We extracted DNA from each sample and constructed a genomic library of Tibetan fox. Fifteen loci sifted by concentration technique were in Hardy-Weinberg equilibrium, and no significant linkage disequilibrium was found between all the pairs of these fifteen loci after a follow up analysis. Consequently, these fifteen loci qualified as identifying molecular markers. The average number of alleles at these15loci is10.13and average PIC value is0.6440, which suggested a higher content of polymorphism information. Software of Cervus3.0and Structure2.3.2were sued to do individual identification analysis and classified analysis on the results of amplification classification on genes of36Tibetan fox individuals with the help of these15loci. These36individuals were distinguished and parted into two groups successfully, and it fitted that samples were collected from Tibet Ali area and Siqu County in Sichuan. Therefore, these loci are able to provide more genetic diversity information of a population, and they will play an important role in the further research of population structure and trends in future.