Preparation Properties And Detection Application Of Molecular Imprinted Polymer Microspheres For Separation Of Drug Ingredients

Molecular imprinting technology (MIT) has been a hot issue to investigate because which can provide the polymers that have high recognization and selectivity properties to objective molecular. On the base of the review to the progress of MIT, following experiments were carried on.1 using naringin as template molecule, molecularly imprinted polymeric microspheres are synthesised by a one-step swelling and polymerization method. The factors affecting morphology of MIPMs were studied. The result indicate that surface pore size of MIPMs decreases with the increase of the cross linker and the uniformity of microspheres are increase with the decrease of the amount of dibutyl phthalate. The binding ratio of naringin molecule to functional monomer molecule is 1:2 in the certain concentration range. The adsorption and recognition performances of MIPMs for template molecule were studied by UV spectroscopy, infrared spectroscopy, a balanced combination of methods and scatchard analysis. The interaction between template molecule and functional monomer were investigate. That provides a theoretical basis and experimental basis for further study of MIPMs.2 The honokiol molecularly imprinted polymeric microspheres have successfully been prepared in aqueous system by a one-step swelling and polymerization, which be used to separate and purify honokiol in magnolia. Scatchard analysis showed that there were two classes of binding sites formed in the imprinted microspheres, and the equilibrium dissociation constant of binding sites the higher affinity was estimated to be 0.13 mmol·L-1, density of the calculated maximum binding sites was 45.21μmol·g-1. The resultant honokiol imprinted microspheres showed the predominate honokiol recognition coefficient, which was 1.85 times higher than that for the magnolol recognition. MIPMs exhibited specific affinity and good selectivity to the template honokiol, and high purity of honokiol was obtained from the extraction of magnolia. This provides a new idea to separate honokiol from herbs.The present work have great significance on the further research of chromatography, solid phase extraction, membrane separation use molecular imprinting technology.