Sio <sub> 2 </ Sub> Mesoporous Materials Preparation And Applications In The Biomedical Field

1. The preparation and heavy metals absorption property of functionalized mesoporous silicaMesoporous silica were prepared via the process in which with cationic surfactant (CTAB) and three block copolymer surfactant (P123) as the templates, then sulfhydryl modified mesoporous silica through Xylene reflux method. The structure and morphology of the materials were characterized by TEM, SEM, FT-IR, XRD, Surface area analyzer, and the character of the materials before and after adsorb heavy metals were investigated by ICP-AES, the result show that with the increasing of the PH, the final effect is better, especially when the PH=7; Comparing the modified and unmodified materials, the former have a much better adsorb effect than the latter, and the sulfhydryl modified mesoporous silica can not only have a higher bio-security but also have a selective adsorption ability.2. The preparation and application in the biological field of SiO2 core-shell structure/mesoporous nano materialCore-shell structural Fe3O4@SiO2 nanocomposite particles were prepared via a sol-gel process, in which the hydrolysis of TEOS was conducted on the surface of Fe3O4 under the aid of basic catalyst. The amino-modified Fe3O4@SiO2 nanocomposite materials were prepared via pre-graft method at the water bath temperature of 40℃,in which APTES was the coupling agent; The structure and morphology of these particles were characterized by infrared spectrum and transmission electron microscopy, and the adsorption property of these core-shell particles before and after amino-modified about plasmid DNA was investigated by Gel-electrophoresis and Bio-spectrophotometer, the result show that:the amino-modified nanocomposite materials have a better adsorption performance about the plasmid DNA than the unmodified materials, the adsorption capacity and adsorption rate of modified materials were improved; Moreover, with the increasing of the amount of materials, the final effect is better, and because of the good magnetic properties of materials, the DNA which was adsorbed can be recycled more efficient and more convenient.Besides, on the basis of the above experiment, nano mesoporous silica with different forms were prepared via the process in which with two of the specific surfactants as templates, these templates include CTAB, F127, P123 and CTAC; The forms of these nano mesoporous silica can be described as worm channels form, short beam form and long beam form. Mesoporous material can obtain magnetic character via deoxygenize after immersed in metal salts or coat Fe3O4 nano particles through sol-gel process; Then with these materials as drug carrier model, modified their surface, and graft specific bonds(such as -NH2); The bio-transfection experiment and targeted simulation experiment show that the transfection efficiency of these materials was involved with size, and these materials can not only have a high bio-security but also can achieve the effect of magnetic targeted therapy.