The Synthesis, Characterization And Application Of Terbuthylazine And Diclofenac Imprinted Polymers

Molecularly imprinted technology is a new-emerging polymerizing technology, which can produces molecule recognition materials with high selectivity. The use of imprinted polymers in several analytical techniques, including liquid chromatography , capillary electrophoresis and capillary electrochromatography, solid phase extraction, mimic biosensors pharmaceutical analysis,enzyme mimic and immunoassay, have been investigated. In this thesis, diclofenac and terbuthylazine were synthesized by situ polymerization, terbuthylazine synthesized by bulk polymerization. Research on the recognition principium, synthetic conditions,and the effect to adsorption characteristics of molecularly imprinted polymers. There are four chapters here:Chapter one, The principle of molecular imprinted polymers, application of this technique and its future developments were introduced.Chapter tow, A series of molecularly imprinted monolithic columns were synthesized using terbuthylazine as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, toluene and dodecanol as the mixed porogenc solvents by in situ thermal-initiated copolymerization technique. The infiuence of porogenic composition, monomer–template ratio and polymerization conditions on the chromatographic performance was investigated. Finally, the recognition performance of the imprinted monolithic column was investigated. Terbuthylazine and its analogues were baseline separated on the monolithic column. The results showed the best ratio of template to functional monomer was l:4,and the toluene content in porogen was 20%. The separation factors of tebuthylazine,simazine,atrazine reaehed 3.01,2.60,2.60. The pore properties of imprinted and non-imprinted polymer were also determined and the results showed different pore distribution.Chapter three, The molecularly imprinted polymers for terbuthylazine was synthesized, it showed a special binding ability for the template and some adsorbability to three other analogues. The polymers can meet the requirements for determination of terbuthylazine in water. Scathcard analysis showed that two classes of binding sites existed in the apigenin imprinted polymers, with their K_d and Q_max estimated to be 1.12, 23.31 mgL^-1; 0.81, 2.17 mg^-1 respectively. Molecularly imprinted polymer showed the higher affinity than non-imprinted polymer. The average recovery was 93.80%,92.50%,89.30% respectively and the relative standard was less than 5%.Chapter four, A series of molecularly imprinted monolithic columns were synthesized using diclofenac as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, toluene and dodecanol as the mixed porogenc solvents by in situ thermal-initiated copolymerization technique. The infiuence of porogenic composition, monomer–template ratio and polymerization conditions on the chromatographic performance was investigated. By adopting low polar porogenic solvents of toluene and dodecanol and optimal polymerization conditions, the molecularly imprinted monolithic column with good flow-through properties and high resolution were prepared. Finally, the recognition performance of the imprinted monolithic column was investigated. The result showed that the stationary phase obtained from the optimization conditions could completely separated diclofenac and its analogues in 15 min with separation factor of 1.54.