The Synthesis And Characterization Of Metsulforon Methyl And Emodin Imprinted Polymers

Molecularly imprinted technology is a kind of new-emerging polymerizing technology, which produces molecule recognition materials with high selectivity and affinity. Polymerization is occurred between functional monomers and crosslinker around template molecules. After extraction of the template molecules, molecular imprinted polymers (MIPs) with specifically memory to the template are obtained. Molecularly imprinted technology has become more and more popular in recent years. MIPs have been widely applied in environmental analysis, pharmaceutical separation and analysis, enzyme mimic catalyst and mimic biosensors etc.In our research, we synthesized metsulforon methyl and emodin imprinted polymers employing non-covalent imprinted technology by bulk and in-situ polymerization respectively. The binding capability and recognition mechanism were characterized by different methods. The research in this thesis include:1) The progress of molecularly imprinted technology, basic theory, synthesis, application, and future prospects of molecularly imprinted polymer were reviewed. The synthesis methods and the factor of influencing the recognition property of molecularly imprinted polymers were emphasized.2) In this thesis, the molecularly imprinted terpolymer were synthesized usingα-methacrylic acid and 4-vinylpyridine as co-functional monomers, ethylene glycol dimethacrylate as crosslinker, azobisisobutyronitrile as initiator, chloroform as porogenic reagent and metsulfuron methyl as template molecules by bulk polymerization. The binding capacity and selectivity of MIPs in organic and water systems were evaluated by solid phase extration. The binding capacity of terpolymer and bipolymers were tested and concluded that the former has higher capacity than the other two polymers, this is very useful for the recovery from the waste of metsulfuron methyl factory. Further we employ the Proton Nuclear Magnetic Resonance (1H NMR)titration test to explore the phenomenon of high capacity and the results indicated that there was obvious hydrogen bond interaction between MSM and the co-monomers of methacrylic acid and 4-vinylpyridine, this indicated that the stronger sites were formed.3) The molecularly imprinted monolithic columns were synthesized usingα-methacrylic acid as functional monomers, ethylene glycol dimethacrylate as crosslinker, azobisisobutyronitrile as initiator, the mixture of toluene and dodecadol as porogenic reagent and emodin as template molecules by in-site polymerization. From the capacity of emodin in monolithic column, we can see that if the template showing stronger retention on non-imprinted polymers will give stronger retention on corresponding imprinted polymers. On the basis of the theory, we optimized the synthesis conditions of monolithic column and the polymer synthesizing in the optimum condition can separate the emodin and its analogues successfully.