Synthesis, Antimicrobial Study Of Platium (Ⅳ) Complexes With Compounds Containing N Groupand And The Interaction Between The Complex And DNA

Three binary platinum complexes were first synthesized, with ligands of imidazole, 2,2-biimidazole and phenanthroline, respectively, then introduced a second ligand, obtaining platinum-phenanthroline ternary complexes. There chemical composition were determined by elemental analysis, molar conductance, IR, UV-Vis, and thermogravimetric analysis, etc. At the same time, the antibacterial activity of three kind of binary complexes and the interaction between five kinds of complexes and DNA were studied systemally. This paper includes the following four parts.1. SummaryWe first introduced the progress of metal complexes in anti-cancer and anti-bacterial field and summarized the study of nitrogen-containing heterocyclic compounds and nitrogen-containing heterocyclic complexes. On this basis, we established our research. And then the interaction of metal complexes and ct-DNA and research methods were also generalized. Finally, we elaborated design ideas and research significance of this subject.2. Synthesis, Characterization and Antimicrobial study of nitrogen-containing heteroc-yclic platinum (Ⅳ) ComplexesWe synthesized three platium(Ⅳ) complexes with Compounds Containing N Groupand, which chemical composition were determined by elemental analysis, molar conductance, IR, UV-Vis, thermogravimetric analysis, and HNMR, speculated their structures, and also studied their antimicrobial activity. The results showed that the antimicrobial activity of platinum (Ⅳ) imidazole complexes and 2,2 '- imidazole (Ⅳ) complexes to E. coli, Staphylococcus aureus and Bacillus subtilis were significantly stronger than that of the corresponding ligand. Especailly to E. coli, the two complexes showed broad-spectrum antimicrobial activity; phenanthroline platinum (Ⅳ) complexes have some antibacterial activity, but compared with the ligands, their antibacterial activities have no much difference. 3. Interaction of nitrogen-containing heterocyclic platinum (Ⅳ)binary complexes with DNAWe investigated the interaction of complexes and ct-DNA by UV absorption and fluorescence spectroscopy, Ethidium bromide as a fluorescent probe, explored the characteristic of fluoresence quenching and caculated binding constants, binding sites n and thermodynamic parameters of these compounds with DNA-EB system. We speculated the main binding mode of the three nitrogen-containing heterocyclic platinum (Ⅳ) complexes with DNA by thermal denaturation and ionic strength experiments. The interaction between heterocyclic platinum(Ⅳ) binary complexes and DNA is strong and stable, in the mode of intercalation. And the intercalation between heterocyclic platinum (Ⅳ) binary complexes and DNA can make DNA double helix destructed, which leads to the antibacterial activity of these complexes, and it provided theoretical basis for designing a low toxicity, efficient platinum (Ⅳ) anti-tumor complexes.4. Synthesis, Characterization and Interaction with DNA of heterocyclic platinum (Ⅳ) Ternary ComplexesWe synthesized two heterocyclic platinum (Ⅳ) ternary complexes, Pt(Ⅳ)-phenant -hroline-oxalic acid and Pt (Ⅳ)-phenanthroline-glycine, determined their chemical co -mposition and structure by elemental analysis, molar conductance, IR, UV-Vis and th -ermogravimetric analysis, and studie -d their interaction with DNA. The research m -ethod is the same as that of binary complexes. The results implied that two kinds of ternary complex complexes with DNA also have strong and stable effect via intercalat intercalation, And the binding constant between them are bigger than that of the corre -sponding platinum (Ⅳ) phenanthroline complexes and DNA, further indicate that the second ligand enhance the integration between ternary complexes complexes and DNA is greater.