| The nitrogen removal of production, sanitary and animal waste and the ammonia gas removal of the atmosphere were the present research hotspots of sewage disposal and air purification. This research were mainly focused on the transformation of ammonium nitrogen by nitrobacteria, the convertion of ammonium nitrogen in the water and ammonia gas inthe atmosphere by immobilized nitrobacteria were also studied. The results were mainly described as follows:1. The amount of the nitrobacteria in the soil and their transformation of ammonium nitrogen indicated that:①There were obvious difference among the amounts of the nitrobacteria from different soil samples, The amount of the nitrobacteria highly reached 1.51×106 cfu/g dry soil, 30 times as much as the lowest ones.②Under the controlled condition, the tested soil nitrobacteria displayed great convertion ability of ammonium nitrogen in CMINB culture. when cultured for 20 d, the convertion ratio were 33.8% ~ 69.9%.③There were relativity existed between the percent convertion of ammonium nitrogen in CMINB culture by nitrobacteria and the soil nutrient content. Significant differenc(eP<0.01)were observed between the percent convertion of ammonium nitrogen and available K on 11.5d and 20 d; and the relationship between the NH4+-N conversion ratio and the content of available P was significant;Significant difference(P<0.05)were also obtained between the percent convertion of ammonium nitrogen and ammonium nitrogen content in the soil. The results indicated that nitrobacteria, which were isolated from the high available nutrient content soil, had great ability of ammonium nitrogen transformation; the ammonium nitrogen transformation nitrobacteria may be survival in such soils .2. The research about the impact factor of ammonium nitrogen transfomation by nitrobacteria demonstrated that:①Ventilate to the culture promoted the transformation of ammonium nitrogen by nitrobacteria, and the difference of the ammonium nitrogen conversion ratio among the 4 h/d treatment, the 1 h/d treatment and the control treatment was significant.②the ammonium nitrogen conversion ratio decreased 11.3% ~ 76.0% by using NaOH to adjust the pH, and the ammonium nitrogen conversion ratio was 148.1% higher in the treatment by using 0.5 g/L Ca(OH)2 to adjust pH than that in the treatment by using 2.5 g/L CaCO3.3. The study of the effect of INB on the conversion of ammonium nitrogen indicated: INB made by the bricks and china as the immobilized carrier had the ability of transformation of the ammonium nitrogen in water. The conversion ratio of ammonium nitrogen by the INB made by bricks was 36.9% after 23h treating by 2.43 g/L (NH4)2SO4, and the conversion ratio of ammonium nitrogen by the INB made by china was 5.6% after 4d treating by 2.5 g/L (NH4)2SO4.4. INB made by vermiculite could transform ammonium nitrogen in water.①The conversion ratio of ammonium nitrogen was 15.0% after 4h treating by 20 g/L (NH4)2SO4.②the amount of INB made by vermiculite affected the ammonium nitrogen conversion ratio. The ammonium nitrogen conversion ratio were 3.6%, 6.9%, and 9.7% respectively by adding 5 g, 1 0g and 20 g after 4 h treating by 5.00 g/L (NH4)2SO4 . While the ammonium nitrogen conversion ratio were 8.1%, 10.4% and 15.0% treating by 20 g/L (NH4)2SO4.③Temperature was another impact factor of the conversion ratio. The ammonium nitrogen conversion ratio were 8.6%, 9.6% and 12.1% respectively at 20℃, 30℃and 40℃, after adding 20 g INB and being treated 4 h by 5.00 g/L (NH4)2SO4.④The medium volume in the closed incubation affect the ammonium nitrogen conversion ratio. After treated for 4h by 5.00 g/L (NH4)2SO4, the ammonium nitrogen conversion ratio were 5.6%, 7.9% and 10.5% respectively as the medium volume were 70, 140 and 280 ml.⑤The ammonium nitrogen conversion ratio increased as the reacting time increased. After adding 20g INB, treating by 5.00 g/L (NH4)2SO4, the ammonium nitrogen conversion ratio were 11.6%, 11.8% and 14.1% respectively when the reacting time were 2 h, 4 h and 6 h.5. The transformation effect of INB on NH3 showed that:①INB made by the bricks and china as the immobilized carrier displayed great atmosphere ammonia gas removal effect. The conversion ratio of the tested immobilized nitrobacteria reached 67.3%, when ammonia gas got access to the INB for 10 h; 35.8% were obtained after 5 h;②The convertion ratio of ammonium nitrogen by INB made by the bricks coule be improved via the multiplication regeneration.The conversion ratio of immobilized nitrobacteria on ammonia gas were improved by 27.5%, when INB were accumulation cultured at 37℃for 12 h. The conclusion could be obtained that, the common building brick and vermiculite could be used as adsorptive support for immobilized nitrobacteria, especially the brick carrier, which showed high convertion ability of ammonia gas;The conversion ratio could be increased by adding the culture media regularly and accumulation culture, in order to achieve the recycle of the INB. |
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