| Cordyceps militaris industry developed rapidly in our country, there are still the following drawbacks:(1) C.militaris artificial propagation of the current low level of standardization, the lack of systematic cultivation of technical specifications, especially for the bacteria, the growth parameters less research. (2) deep processed products Cordyceps few studies on the development and utilization of Cordyceps is more rare. In this study, the above situation to carry out targeted research and development, to form the following conclusions, in order to standardize the production and depth of the development of Cordyceps provide a reference.This study was Developed C.militaris strains standards to ensure the quality of the selected strain, followed by the formation of the C.militaris production technology specification, and its technical points for the bacteria to the fat out of the grass temperature range between 20～25℃, optimum temperature 20～23℃, the formation of the primordium stage and out of grass also need to 24h light pre-light stimulation and ventilation from time to time to ensure the oxygen supply. Suco pupae of their own species No.1 was tested to cultivate, its fresh products of biological conversion rate can be up to 50%.This study, a systematic comparison of the C.militaris(Sukeyong nol)and the composition and content of the wild Cordyceps sinensis, Cordyceps militaris fruiting bodies found in the health and nutritional composition and close to wild Cordyceps sinensisThis study optimizes the hot water extraction method of Cordyceps polysaccharides extracted optimize the design of orthogonal extraction of polysaccharides was found in 90℃, water 20 times, one time extraction, each five hours as the best extraction conditions. The use of anthrone-sulfuric acid method for the determination of polysaccharide C. militaris conducted Cordyceps Polysaccharide among proteins and found that a higher rate of Sevage method of protein removal, and its highest rate of sugar retention; calcium chloride laws, due to low-cost, simple operation, removal of protein and sugar retention can still be effective. Trichloroacetic acid method and hydrochloric acid method of removing protein despite good results, but the sugar retention rate is low, therefore, Cordyceps polysaccharide explore new and efficient separation technique is also very important next step the focus of research work carried out.(4) In this study, microwave extraction cordycepin new technology, optimize the use of orthogonal cordycepin microwave extraction process, was established in Cordyceps militaris by HPLC. For the extraction of cordycepin and product development provides a means of quantitative analysis.(5) using seven kinds of macroporous resin adsorption in Cordyceps militaris purified, selected the appropriate resin AB-8, studied factors such as pH value of the static adsorption of the resin, and the eluant methanol volume fraction of the static desorption effect, while also studied the dynamics of cordycepin adsorption and desorption curves. The results showed that:AB-8 resin in Cordyceps militaris has a pretty good adsorption purification performance of the sample solution when the pH value of 5.0; adsorption temperature 20℃; absorption liquid mass concentration of 0.15 mg/ml; the sample fluid volume of 10 times the volume of resin column; adsorption flow rate 1.8BV/h when the adsorption of the best; elution process conditions to 2.5 times the resin column volumes of 15% methanol. |
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