Utilization Of Echinocandin B Fermentation Waste

China is a major producer of antibiotic product. With the increasing output of antibiotics, the fermentation waste is becoming a big problem. The production wastewater and fermentation residue are two of the major pollutant of the antibiotic waste which contain residue antibiotics and other substance difficult to treat. In China, echinocandin B, as a clinically important anti-fungal drug, its producing process is being extensively studied. Waste treating technology is one of the key steps of its clean producing process.The fermentative waste of Echinocandin B is mainly consisting of Aspergillus nidulans mycelia and residual medium, so is rich of bacterial proteins, carbohydrates, nucleic acids and other nutrients. In this study, the echinocandin B residue is used as alternative medium of nitrogen in the production of different kinds of antibiotics. The results showed that the fermentation unit of echinocandin B has declined remarkably with the alternative nitrogen. However the fermentation units of geldanamycin and ramoplanin are not the same. When the replacement amount of soybean powder is less than 80%, the potency of geldanamycin were able to reach the control level. When the replacement ratio of soybean powder is 20%, the potency of geldanamycin was 21% higher than control. Ramoplanin potency is 32% higher than that of control with 20% replacement ratio of yeast extract in the medium. Antibiotic fermentation residue will be used as a substitute of nitrogen source for antibiotic production. It is not only a new way to treat sludge, but also cut the cost of antibiotics producing. And it is environmental-friendly and more economic.Anaerobic fermentative technology is widely used in the treatment of antibiotic industrial wastewater and organic waste. This approach can not only achieve waste reduction, sterilization effect, but also have potential of degrade complex compounds or toxic substances. However, there's no report about anaerobic biological treatment of antibiotic fermentation residue. To this end, this study examined the anaerobic treatment of wastewater and fermentation residue of echinocandin B to provide a new treatment approach and technical support. By adjusting the inoculums and wastewater ratio to 4:1, the reactor runs well. At day 13, the digestion was nearly droved to the end, the COD removal rate reaches 73.3%, the methane content is above 70%, the total biogas production is 5.1 L and with 3.0 L methane in it.Anaerobic biological treatment of fermentation residues of echinocandin B showed that sludge digestion can produce biogas, but the gas production and methane content were significantly lower than the result of digestion of pig manure and rice straw as substrate. Screening by using a transparent ring method, we obtained a chitinase producing Actinomycetes G8. Shake-flask experiment indicates that G8 gains great cell wall degradation capacity of the Aspergillus nidulans. Preliminary study of different carbon sources on the G8 enzyme activity of chitinase and its basic characteristics, expectations through enzymatic digestion of the echinocandin B fermentation residue to release the residual antibiotic and other intracellular materials to improve the efficiency of their anaerobic digestion.