| In recent years, Plasticizer in plastic packaging effects on human health has become a focus of attention.This paper research into plasticizer bisphenol A, Select baby bottles,polycarbonate cups,disposable lunch boxes as study object, using flow injection chemiluminescence detecting bisphenol A in which, This paper aims to research on rapid detection method of bisphenol A, which is significant of food safety oversight and supervision.After the samples in this paper extracted by C18 solid-phase extraction column, detected bisphenol A content in them by flow-inject Chemiluminescence.Details are as follows:Weigh 2 g (accurate to 0.0001 g) samples in 200mL beakerflask with stopper, added high-purity water, 2h after ultrasonic extraction under 80℃.Using C18 solid phase extraction column, the extraction conditions is: using 3 mL of methanol, 3 mL high-purity water for activation, Pipette be purified liquid to solid phase extraction column, using 3 mL of high-purity water and 3 mL of methanol elution and drained, with 3 mL methanol elution, collecting eluate. The elution was dried with nitrogen at 50℃, then residuum was dissolved in absolute alcohol .Bisphenol A was detected by luminal-H2O2-Cr3+ chemiluminescence Optimum conditions of this chemiluminescence system were pH12.00 luminol solution, 5.0×10-4mol/L,H2O2 4.0×10-2mol/L , Cr3+1.0×10-5 mol/L, pH2.50 BPA test solution. Under this condition standard curve was drawn. The result indicated that it presented good linear relationship when the contents are in the range of 2.0×10-9 mol/L～1.0×10-5 mol/L,related coefficient was 0.9986, etection limit was7.22×10-10mol/L, RSD was 2.5%, Bisphenol A liquid and samples were dtermined under the condition, detection extraction recoveries were over 92%.Bisphenol A was detected by Luminol-potassium permanganate chemiluminescence system Optimum conditions of this system were pH12.50luminol solution 6.0×10-4mol/L, KMnO4 2.0×10-4mol/L, pH3.00 BPA test solution, Under this condition , the linear range of bisphenol A was 1.0×10-11～1.0×10-5mol/L, detection limit was 4.26×10-12mol/L, RSD was 1.8%, For the determination of bisphenol A,with satisfactory results.Controlled trial carried out by HPLC, Chromatographic column :symmetry300 C18 5μm, mobile phase :Methanol + water=70+30, Column temperature: room temperature, Fluorescence detector: excitation wavelength 227nm, emission wavelength 313 nm. The lineat range was 1.75×10-4mol/L～1.32×10-7mol/L, the detection limit was 2.94×10-9mol/L, RSD was 2.0%.Compared with the national standard, Luminol-potassium permanganate flow-injection chemiluminescence system has low detection limit,high sensitivity,low-cost and elatively stable system.It is an ideal rapid new analysis method of determing bisphenol A in food contact materials. |
PDF Full Text Request