Study On The Synthesis And Absorption Characterization Of New Molecularly Imprinted Polymer

Molecular Imprinting Technique (MIT) is becoming increasingly recognized as a powerful technique of preparing synthetic polymers that contain tailor-made recognition sides for certain molecules, which is called molecularly imprinted polymer(MIPs). The MIPs shows the superiority in separation, purification, biological modeling, immunoassays and trace analysis for its predetermination, specificity and practicability. It also has an extensive application.This paper gives a comprehensive review of the basic principle of molecular imprinting technique, molecularly imprinted polymer preparation methods, application field and characterization method, and also introduces preparation method of the magnetic composite imprinting microspheres. The existing problems and future development trends are analyzed and predicted. This research is divided into three parts. The micron-grade polystyrene microspheres are prepared by soap-free emulsion polymerization which are used as seed for preparing Gatifloxacin, molecularly imprinted polymer using a single step swelling polymerization. The monodisperse silica particles are prepared by Stober method, then modified byγ-methacrylic propyltrimethoxysilane(MPS) as a coupling agent, and we prepared SiO₂-St composite particles through dispersion polymerization method, using modified particles as seed, finally the Rotenone molecularly imprinted polymer is synthesised using SiO₂-St composite microspheres as seeds. The magnetic molecularly imprinted polymer is prepared with Fe₃O₄ particles for magnetic components, tyrosine as template molecule, mixture of MAA and AM as functional monomer. And we study adsorption behavior of polymer and molecular recognition performance. The poymer is characterized by several method with scanning electron microscope, ultraviolet spectrum, infrared spectrum and fluorescence spectrum method.The research shows that Gatifloxacin molecularly imprinted polymer microspheres prepared in the water phase using polystyrene microspheres as seed can combine three functional monomer, MIPMs is in favour of contacting substrate and binding sites with wide size distribution, rough surface and porous structure. Ssatchard analysis indicate there are two kings of binding sites existing in the recognition process. And the Rotenone molecularly imprinted polymer with SiO₂-St as seed reveal better adsoption ability and good regeneration properties in equilibrium adsorption separation experiments. The research also shows that the magnetic molecularly imprinted polymer is spherical, agglomerative state and wider particle size distribution, which has good adsorption performance with existing caities in surface. And the polymer can be quickly separated in additional magnetic field. With phenylalanine as competitive substrate, the imprinting factor of 1.87 relative to tryptophan is significantly higher than the imprinting factor of 1.03 relative to phenylalanine. The result explain the imprinted polymer has very good recognition performance and higher adsorption capacity for specific template molecule.