| Silver ion is known to a transition metal which can bind with various metabolites leading to inactivate enzymes and hamper metabolism, and it has been detected in more and more biology. Thus, development of high sensitive and selective methods for the determination of silver ion is of considerable importance for the environment and human health. The fluorophore based on aggregation-induced emission(AIE) has attracted the interest of analysts because it can recuperate the disadvantages of traditional aggregation-caused quenching (ACQ).Three target molecules were designed and synthesized in this thesis and the structures of the products were characterized by 1H NMR,13C NMR,ESI-MS. Their optical properties of response to Ag+ were systematically determined and studied by ultraviolet-visible spectrum and Fluorescence spectrum.The sensor M1 with two BPA units, attached on TPE fluorophore, was able to recognized Ag+ selectively in THF/H2O (1:2, v/v) solution with the presence of other metal ions except Hg2+,Zn2+ and Cu2+ which interfere with the M1-Ag complex weakly. With the addition of Ag+, the Fluorescence spectrum shows remarkable bathochromic shift with 50 nm. Moreover, the fluorescence intensity of M1 increases linearly with the concentration of Ag+, where the linear dependency coefficient is 0.997, and the detection limit of this Ag+ assay can reach 1μM. M1 is insensitive to pH and can detect Ag+ with a wide range of pH, which make it more widely applications. The research result of the fluorescent enhancement of Ml indicate that the BPA units and triazole moieties were combined to Ag+ by two different modes, and when the concentration ratio of Ml/Ag+ reaches to 1:2, M1 was caused to aggregate leading to emission. Thus, M1 can be used as a predominant fluorescence probe based on fluorescent enhancement for Ag+.M2 and M3 were discovered to behave excellent AIE property, but no response to Ag+, so M2 and M3 are not suitable as Fluorescence probes. |
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