The Application Of New-Style Prussion Blue Complex Nanomaterial In Electrochemical Biosensor

In recent years, the composite nanomaterials have attracted steadily growing attention due to their interesting electrochemical properties and good biocompatibility. Composite nanomaterials were normally modified on the electrode surface using different methods to show excellent electrochemical activity. Therefore, the composite nanomaterials have potential prospect in constructing electrochemical sensing and biosensing platforms.In this paper, the three different types of the prussian blue (PB) composite nanomaterials were fabricated on the modified electrode to make use of chemistry and electrodeposition method, and a series of research were listed as follows:(1) Potentiostatic electrodeposition was adopted to fabricate PB nanoparticles, which were coated on the reduced graphene oxide (RGNO) modified carbon paste electrodes. The characteristics of the modified electrodes were investigated by electrochemical impedance spectroscopy, cyclic voltammetry, and differential pulse voltammetry, respectively. Two RGNO, namely electrochemically reduced graphene oxide (ERGNO) and chemically reduced graphene oxide (CRGNO), were selected to prepare the platforms for PB electrodeposition. The two methods of reduced graphene oxide were compared and the time of electrochemical reduced, the time of potentiostatic electrodeposition, and so on were optimized. The results showed that ERGNO exhibited better electrochemical performances and more affinity for PB electrodeposition than CRGNO. Using the PB/ERGNO-modified electrode as an electrocatalytic platform, a high performance DNA biosensor was constructed and achieved the simultaneous determination of guanine and adenine.(2) Direct electrochemistry of guanine and nonenzymatic electrochemical H₂O₂ detection were investigated on PB/IL and PB/MWCNTs composite nanomaterials modified glassy carbon electrode. PB/IL and PB/MWCNTs composite nanomaterials were prepared according to a simple method dripping with the surface of the electrodes. The morphologies and electrochemical properties of the PB composite nanomaterials were characterized by using scanning electron microscopy, cyclic voltammetry, differential pulse voltammetry, amperometry, respectively, and the experimental conditions were optimized by using cyclic voltammetry. Due to the specific chemical characteristics of IL and MWCNTs, the fabricated electrodes showed good electrochemical performances to different electroactive molecules. The experiments suggested that the PB composite nanomaterials modified electrodes showed good electrochemical signals and revealed the sensitive detection of guanine and H₂O₂ successfully.