The Research On Extraction Protein And Biological Activity Of Hydrolysate Derived From Camellia Oleifera Abel Seed Meal

Camellia oleifera Abel belonging to the Theaceae family, small evergreen tree, is one of the main oil crops in China. According to statistics, every year more than1000000tons from Camellia oleifera Abel seed, Camellia oleifera meal, a by-product meal an average annual production of about683900tons, but the effective utilization of Camellia oleifera seed meal is low, causing a great deal of waste. Protein content in seeds of Camellia oleifera is15%-20%, can be used as a new type of plant proteins, through research reports in recent years, Camellia oleifera Abel meal protein peptides have lower blood pressure, immune and antioxidant function, thus becoming the hot research areas of medical and functional food science.This thesis for the extraction of raw materials from Camellia oleifera Abel meal protein, study on extraction of protein of defatted Camellia oleifera route after pretreating by cellulose enzyme.The results showed that the best hydrolysis technics combination of oil-tea-seed is enzymes and substrates ratio for2.67%, hydrolysis temperature for43.67℃, hydrolysis pH is10.41, follow-up treatment after hydrolysis conditions of Camellia oleifera meal protein extraction rate of70.36%, compared with the cellulose enzyme pretreatment is not in use (protein extraction rate64.97%) has improved significantly. With Camellia oleifera meal protein as raw materials, the hydrolysis condition was optimized through response surface method (RSM) with pH value (X1), hydrolysis time (X2, h) and hydrolysis temperature (X3,℃) and the enzyme and the substrate (X4,%) as its experimental parameters, with ACE inhibitory activity of Camellia oleifera Abel meal protein hydrolysate in response value. The results showed that the best hydrolysis condition is the optimum pH value8.1, enzyme and substrate ratio of1.57%, the best temperature at50.5℃and the best hydrolysis for6.66h. Under these conditions, Camellia oleifera meal peptide prediction model of ACEI is86.17%.Through feeding SHR Camellia oleifera meal of alkaline protease hydrolysis polypeptide (MW<3kDa), we research on its antihypertensive effects. The SHR high, medium and low dose groups, respectively gavage500mg/kg·bw,250mg/kg·bw, and100mg/kg·bw, Minimum value occurs after intragastric4h, the overall trend is u-shaped, where different dosage groups and the positive control group significant differences in effects on rat (P<0.05). Long-term medication after9weeks of Camellia oleifera meal protein hydrolysate, compared to the blank control group, the positive control group and dose group are marked reduction in systolic blood pressure of SHR (P<0.01), and with the extension of the delivery time, systolic blood pressure of essential hypertension rats on the decline slowed and stabilized, but had no significant difference on the SHR weight (P>0.05).As Kunming mice is test object, established by injection of cyclophosphamide immunodepressed model for immune suppression group, with high, medium and low dose (500mg/kg· bw,250mg/kg· bw,100mg/kg· bw) of Camellia oleifera meal peptide feeding mice, the index of thymus and spleen in high, medium, low dose groups mice have increased significantly, and is associated with the polypeptide dose of high-dose medium-dose groups can dramatically restore immunity caused by injection of cyclophosphamide lower index of thymus and spleen index in mice (P<0.01). Camellia oleifera meal peptide high dose group has a strong promotion of phagocytosis of activity (P<0.01), the delayed-type hypersensitivity in mice induced by DNFB enhancement (P<0.05), can significantly improve the DNA content in mouse bone marrow cells (P<0.05), effective promotion of antibody activity (P<0.05), effects of a index test and delayed-type hypersensitivity in mice trials apparent dose-response relationships.