Preparation Of Rice Protein From Rice Dregs And The Study On High Fischer Value Oligopeptide

Rice dregs is the by-products of rice after it is fermented to produce monosodium qlutamate,alcohol, lactic acid, starch sugar, which content protein as high as 50% to 60%, it can be used as preparation of protein powder, all kinds of food additives, or prepared with a variety of physiological activity of the peptide. But at ptesdent,native company generally sold it as animal feed, which resulting high-quality protein and more great waste of resources, so how to make use of rice protein, to improve its economic efficiency are very important.High Fisher value oligopeptide means F value higher than 20. Medical experiments show that the high F value oligopeptide has good physiological functions, such as to eliminate or reduce the symptoms of hepatic encephalopathy, fatigue, improve the protein nutrition, ease alcoholism, inhibit cancer cell proliferation, reduce serum cholesterol levels, etc.,it has strong application value.This paper mostly study the rice dregs turned to rice concentrate protein obtained by water wash method; then used as raw material, through two-step hydrolysis, get the high F values obtained oligopeptide and amino acid mixture and adsorption with activated carbon to remove the free amino acids, to preparate the high purity higher F value oligopeptide. Specific contents are as follows:(1) Smash the rice drags, over 100 mesh sieve, using Soxhlet extraction method to remove the fat, and then washed by water to remove soluble substances .In the process, the temperature, the washing time, water ratio are being researched. The orthogonal experiment based on the single factor experiment, the use of SPSS statistical software to analyze the experimental data, the optimal factor level are as follows: temperature 70℃, time 0.5h, water ratio1:10, In these conditions, the resulting contention of protein is 78.4%, after the second washing, protein contention reached to 82.5%.(2) In this research,we adopt RSM to optimize the technics of produce high fisher value oligopeptied.Considering there are too many factors in hydrolyze,and there may existent interrelation,so we calculate these factors totally.Basde on sigle factor experiment,find out six effect factors.then,filter three factors which effect markedly by Plackett-Burman experiment design.They are the time of two enzymes,and pH. Then,use the Box-Benhnken experiment of RSM to analysis these three factors,to find out the appropriate equation.The result shows that the R2 of the equation is 0.9865.Then compute the condition of the three factors from the equation,they are: X1=-0.25, X2 =0.44, X3=-0.15,the time of is 1.75h,the time of is 2.44h,the pH of is 5.85, then, from the condition to calculate the degree of hydrolyze is 33.37%.(3)Use double enzyme hydrolysis mixture of peptides and amino acids as raw materials, select the activated carbon as adsorbent to remove the free aromatic amino acids, also make a bleaching effect. Use OD₂₂₀/OD₂₈₀ as response value. The bigger OD₂₂₀ means there are more AAA in the hydrolyze liquid. The smaller means there are less BCAA in the hydrolyze liquid. So, bigger shows there are more AAA in the hydrolyze liquid related to BCAA. Then use the B-B experimental design of RSM to get the best activated carbon adsorption process conditions: temperature 44℃, time1.07 h, solid to liquid ratio1﹕10, in this condition, OD₂₂₀/OD₂₈₀ reached 50.40.(4)Use the amino acid analyzer to mensuiation the amino acid composition, and then calculate the F value is 37.62;and then dissolved, Utilize the protein purification system obtaining the peptide molecular weight group was divided into: 300Da-500Da components, 700Da-1020Da component. This is entirely consistent with the high F value of the oligopeptide requirements...