Studies On The Preparations And Properties Of Alginate Complex Nano Micelles

Monodispersed complex nanoparticles were synthesized simply by mixing alginate acid (ALG-H) with poly[(2-dimethylamino) ethyl methacrylate] (PDEMA) in pure water. The structure and properties of the nanoparticles were extensively studied. The surface charges and average sizes of the nanoparticles were varied with the composition of ALG-H and PDEMA. The naonparticles were formed through electrostatic attraction, and they were very stable in pure water, but dissociated in salt solutions. An anticancer drug (doxorubicin) was loaded in the nanoparticles (AP55) and released in different saline solutions. The release profiles revealed that the drug release could be controlled by adjusting the pH and salt concentrations.In order to improve the drug loading capacity toward hydrophobic drugs, monomers of (2-dimethylamino) ethyl methacrylate (DEMA) and diacetone acrylamide (DAA) were copolymerized in the presence of alginic acid (ALG-H) in aqueous solution yielding nanoparticles in one-pot. The nanoparticle is composed of hydrophobic P(DEMA-co-DAA) core and hydrophilic alginate shell, and the two segments are connected via electronic interaction between cations of DEMA and anions of alginate. The structure and properties of nanoparticles with different content of DAA were studied. The nanoparticles with molar ratio of ALG-H:DEMA:DAA = 1:1:0.5 (ADD-05) possess spherical morphology with an average diameter of 120 nm in narrow distribution. Micelles of ADD-05 were non-toxic and still very stable after stored for 10 months at room temperature, which was very important to their application. The drug loading and embedding rate of ADD-05 toward hydrophobic ibuprofen reached 35.97 % and 97.12 % respectively, also revealing the obvious pH sensitivity. What was more, the embedding rate of ADD-05 toward anticancer drug doxorubicin reached 87.1%, also revealing pH sensitivity and the obvious slow-releasing effect.The nanoparticles displayed apparent advantages such as simple preparation process, low cost, being free of organic solvents, size controllable, biodegradable and biocompatible. Keywords: Alginate; Nanoparticles; Electrostatic attraction; pH sensitivity; Drug release...