The Research On The Extraction And Separation And Activity Of Polyphenols From Camellia Seeds

Camellia oleifera is one of our unique edible oil species. Polyphenols from Camellia seeds have functions of antioxidant, antibacterial, anti-heart and cerebrovascular diseases, are a class of worth developing natural antioxidants. In this paper, seeds of Camellia japonica, Camellia chekiangoleosa, Camellia polyodonta How and Camellia semiserrata Chi. were used to carry out studies of the polyphenolic compounds from Camellia seeds. There are important reference values and positive practical significances for processing technology improvement and development of Camellia seeds polyphenols. This thesis research on the ultrasound-assisted extraction, separation, purification and antioxidant activity of polyphenolic compounds from Camellia seeds, all the results were showed as following:Response surface method was used to optimize conditions of ultrasound-assisted extraction of polyphenolic compounds from Camellia seeds. The optimization extraction conditions were obtained as following:extract temperature,50℃; ratio of material to liquid,1:38(g/mL); ultrasonic treatment time,40min; volume fraction of methanol,60%and pH7. The yield of polyphenols from Camellia seeds could arrive21.0377mg/g, increased by14.68%than the solvent extraction and10.61%than the microwave-assisted extraction.SPE was used to pre-separate and purify the polyphenolic compounds from4kinds of Camellia seeds. HPLC was used to determine and compare the pretreatment results of different elution solvent. Compared by chromatography, pure methanol is the best elution solvent in SPE.Semi-preparative HPLC was used to separate the polyphenolic compounds from Camellia polyodonta How seeds. Conditions and effects of the method were studied. The "operating conditions of the semi-preparative HPLC were determined as following:flow rate,3mL/min; the injection volume,400μL;10major peaks were collected in the chromatogram.DPPH and ABTS method were used to determine the antioxidant activity of polyphenolic fractions from Camellia polyodonta How seeds. The DPPH·radical scavenging capacity of10of fractions were lower than the vitamin C. The ABTS·+radical scavenging capacity of fraction3was stronger than Trolox, the other9of fractions were lower than Trolox. Since only two of the test methods were used, the results were not comprehensive. Further research work should be done in the future.According to the activity tests, the properties of dried fractions and other factors,5of active fractions from Camellia polyodonta How seeds were separated again, and were determined by NMR identification. The results showed that the purity of collected fractions is not high. There are certain differences with the purity test results made by semi-preparative HPLC. This could be due to an association with differences of filling type and particle size of chromatographic column. Fraction4may be flavonoids, the main compound which accounted for80%can be judged containing2rhamanopyranosyl, a β-D-glucoside and5acetoxy connected to the saccharide ring, but the complete molecular structure can not be determined. According to the conclusions above and results of Li Chun Du et al., we inferred this compound as a kaempferol glycoside derivative.