Research On The Isolation Of Microbial Polysaccharide-producing Pathogen From Cabbage (Brassica Chinensis)

Microbial polysaccharides by microorganisms in the process of growth and metabolism by bacteria, fungi, algae and other microorganisms in different environmental conditions metabolic process produces a polysaccharide material. Microbial polysaccharides safe non-toxic, industrial production and life in many areas of the value of a wide range of applications, such as a flocculant, food additives, coating agents, thickeners, preservatives and packaging materials.Isolation of a Gelatinous Polysaccharide-producing Pathogen G2 from Cabbage (Brassica chinensis),the polysaccharide-producing pathogen was isolated by serial dilution method,The characteristics of morphology, physiological and biochemical showed that G2 belonged to Xanthomonas.This study also carried out optimization of fermentation strains G2, G2 strains to determine the optimum fermentation conditions for production of extracellular polysaccharides: culture time for 4d, the culture temperature is 30℃,, the best carbon source was sucrose, the optimal concentration of 10 g / L, fermentation of the amount of extracellular polysaccharide and has little to do with their inoculation, and then extracellular polysaccharide of physiological activity were studied. The results show that different concentrations of extracellular polysaccharides on seed germination and showed a different effect, low concentration of seed germination under stress conditions is not obvious, but in high concentrations of extracellular polysaccharides seedlings cultivated under a more strong drought tolerance, analysis suggested that the extracellular polysaccharides in the suitable concentration of the seed germination of plants under drought stress has a protective role in enhancing the drought resistance of seedlings.